National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Bioresour Technol. 2014 Jul;164:93-9. doi: 10.1016/j.biortech.2014.04.039. Epub 2014 Apr 21.
The optimal mixed culture model of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris was confirmed to enhance lipid production. A double system bubble column photo-bioreactor was designed and used for demonstrating the relationship of yeast and alga in mixed culture. The results showed that using the log-phase cultures of yeast and alga as seeds for mixed culture, the improvements of biomass and lipid yields reached 17.3% and 70.9%, respectively, compared with those of monocultures. Growth curves of two species were confirmed in the double system bubble column photo-bioreactor, and the second growth of yeast was observed during 36-48 h of mixed culture. Synergistic effects of two species for cell growth and lipid accumulation were demonstrated on O2/CO2 balance, substance exchange, dissolved oxygen and pH adjustment in mixed culture. This study provided a theoretical basis and culture model for producing lipids by mixed culture in place of monoculture.
最佳的产油酵母黏红酵母和微藻小球藻混合培养模型被证实可以提高油脂产量。设计并使用双系统鼓泡式光生物反应器来验证混合培养中酵母和藻类的关系。结果表明，与单培养相比，使用酵母和藻类的对数期培养物作为混合培养的种子，生物量和油脂产率分别提高了 17.3%和 70.9%。在双系统鼓泡式光生物反应器中确定了两种生物的生长曲线，在混合培养的 36-48 小时期间观察到酵母的第二次生长。两种生物在混合培养中的 O2/CO2 平衡、物质交换、溶解氧和 pH 值调节方面表现出协同作用，促进细胞生长和脂质积累。本研究为混合培养替代单培养生产油脂提供了理论基础和培养模型。
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