Olaleye Mary Tolulope, Amobonye Ayodeji Emmannuel, Komolafe Kayode, Akinmoladun Afolabi Clement
Department of Biochemistry, School of Sciences, Federal University of Technology, PMB 704, Akure, Nigeria.
Saudi J Biol Sci. 2014 Nov;21(5):486-92. doi: 10.1016/j.sjbs.2014.06.005. Epub 2014 Jun 30.
In the present study, we investigated the hepatoprotective potential of Parinari curatellifolia Planch (Chrysobalanaceae) in experimental rats in order to ascertain the validity of folkloric claims of its effectiveness in the treatment of hepatic-related disorders. Flavonoid extract of P. curatellifolia seed, PCF (10-, 20- or 30 mg/kg body weight) or silymarin (25 mg/kg), dissolved in corn oil, was administered by gavage to experimental animals once daily for 14 consecutive days before liver damage was chemically induced through the administration of acetaminophen (2 g/kg p.o.) on the 14th day. Hepatoprotection was assessed by analyzing liver homogenate and serum for markers of hepatotoxicity - alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transferase (GGT) and lactate dehydrogenase (LDH) activities as well as prothrombin time (PT). Evaluation of biochemical indices of oxidative stress - level of lipid peroxides (LPO), activities of superoxide dismutase (SOD) and catalase, along with histological assessment of hepatic tissue sections were also carried out. Results revealed that all doses of PCF significantly (P < 0.001) and dose dependently prevented acetaminophen-induced increase in serum activities of hepatic enzymes (ALT, AST, GGT, LDH) and PT. Furthermore, PCF (10- and 20 mg/kg) significantly (P < 0.001) reduced lipid peroxidation in liver tissue and restored the activities of the antioxidant enzymes SOD and catalase toward normal levels. Histopathology of the liver tissue showed that PCF mitigated the toxicant-induced hepatocellular necrosis, reduced inflammatory cell infiltration and enhanced hepatocyte regeneration. The results indicated that P. curatellifolia flavonoids demonstrated remarkable hepatoprotective activity in acute liver injury caused by acetaminophen.
在本研究中，我们调查了柯拉塔梨（Parinari curatellifolia Planch，金缕梅科）对实验大鼠的肝脏保护潜力，以确定其在治疗肝脏相关疾病方面民间说法有效性的真实性。将柯拉塔梨种子的黄酮提取物PCF（10、20或30毫克/千克体重）或水飞蓟宾（25毫克/千克）溶于玉米油中，通过灌胃法每日一次给予实验动物，连续14天，在第14天通过给予对乙酰氨基酚（2克/千克口服）化学诱导肝损伤之前进行。通过分析肝匀浆和血清中的肝毒性标志物——丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、γ-谷氨酰转移酶（GGT）和乳酸脱氢酶（LDH）活性以及凝血酶原时间（PT）来评估肝脏保护作用。还进行了氧化应激生化指标的评估——脂质过氧化物（LPO）水平、超氧化物歧化酶（SOD）和过氧化氢酶活性，以及肝组织切片的组织学评估。结果显示，所有剂量的PCF均显著（P<0.001）且呈剂量依赖性地预防了对乙酰氨基酚诱导的血清肝酶（ALT、AST、GGT、LDH）活性和PT的升高。此外，PCF（10和20毫克/千克）显著（P<0.001）降低了肝组织中的脂质过氧化，并将抗氧化酶SOD和过氧化氢酶的活性恢复到正常水平。肝组织的组织病理学显示，PCF减轻了毒物诱导的肝细胞坏死，减少了炎症细胞浸润，并增强了肝细胞再生。结果表明，柯拉塔梨黄酮在对乙酰氨基酚引起的急性肝损伤中表现出显著的肝脏保护活性。
