Gu Xuefeng, Fu Maoying, Ge Zhijun, Zhan Feng, Ding Yuqin, Ni Huihui, Zhang Wei, Zhu Yanfang, Tang Xiaojun, Xiong Lin, Li Jiang, Qiu Liang, Mao Yuan, Zhu Jin
Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
Department of Anesthesiology, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
Sci Rep. 2014 Oct 15;4:6625. doi: 10.1038/srep06625.
Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.
据报道，黑色素瘤相关抗原（MAGE）-A9在人类癌症的发展中起重要作用。然而，MAGE-A9表达与肝细胞癌（HCC）临床病理特征之间的关联尚未完全明确。本研究旨在检测MAGE-A9在人类HCC中的表达，并探讨其表达与HCC临床病理特征之间的关联。采用逆转录-聚合酶链反应（RT-PCR）、一步定量聚合酶链反应（qPCR）和免疫组织化学（IHC）分析来表征MAGE-A9在HCC细胞系和组织中的表达。采用Kaplan-Meier生存分析和Cox回归分析评估100例HCC患者的预后。结果显示，MAGE-A9在HCC中的表达显著高于非癌细胞和组织。此外，HCC中MAGE-A9蛋白的表达水平与病理分级（p = 0.003）、门静脉侵犯（p = 0.001）、远处转移（p = 0.022）和TNM分期（p = 0.005）相关。Cox回归分析进一步显示，MAGE-A9表达是无病生存（p = 0.006）和总生存（p = 0.022）的独立预后因素。这些数据首次表明，MAGE-A9表达是HCC的一个有价值的预后生物标志物，且MAGE-A9高表达提示HCC患者生存结局不良。
