Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
School of Material Science and Engineering, Tianjin University, Tianjin 300072, China.
Neural Regen Res. 2012 Dec 15;7(35):2784-92. doi: 10.3969/j.issn.1673-5374.2012.35.006.
Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fluorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells (MCF-7). Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.
天然脂质体可以少量穿透血脑屏障和血脊髓屏障。经过转染激活转导蛋白修饰的脂质体可以输送抗生素，用于治疗急性细菌感染引起的脑炎症。聚乙二醇偶联的脂质体具有长期循环的能力。在这项研究中，我们制备了转染激活转导蛋白-聚乙二醇修饰的脂质体，并用异硫氰酸荧光素进行了标记。因此，脂质体具有跨膜、长期循环和荧光示踪的特性。通过与人乳腺癌细胞（MCF-7）共培养，观察其摄取、细胞毒性以及穿透血脊髓和血脑屏障的能力。结果表明，脂质体具有良好的生物相容性，与人乳腺癌细胞共培养时细胞毒性低。脂质体可以通过细胞膜，通过全身循环穿透大鼠的血脊髓和血脑屏障，进入中枢神经系统和神经细胞。这些结果验证了异硫氰酸荧光素修饰的转染激活转导蛋白-聚乙二醇脂质体具有穿透血脊髓和血脑屏障的能力。
