Key Laboratory for Prescription of National Educational Ministry of China, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Neural Regen Res. 2012 Dec 25;7(36):2853-9. doi: 10.3969/j.issn.1673-5374.2012.36.001.
The present study monitored the effect of 2, 10, and 50 mg/L of Panax notoginseng saponin exposure following hypoxia-reoxygenation injury in fetal rat cortical neurons. Results showed that varying doses of Panax notoginseng saponin significantly enhanced the cell viability of neurons, reduced malondialdehyde content, increased superoxide dismutase activity, inhibited mRNA and protein expression of inducible and neuronal nitric oxide synthase, and decreased the release of nitric oxide in hypoxia/reoxygenation injured cells. In particular, 50 mg/L of Panax notoginseng saponin was the most effective dose. These findings suggest that Panax notoginseng saponin can attenuate neuronal oxidative stress injury caused by hypoxia/reoxygenation in a dose-dependent manner.
本研究监测了在缺氧再复氧损伤后，2、10 和 50 mg/L 浓度的三七总皂苷对胎鼠皮质神经元的影响。结果表明，不同剂量的三七总皂苷显著提高了神经元的细胞活力，降低了丙二醛含量，增加了超氧化物歧化酶活性，抑制了诱导型和神经元型一氧化氮合酶的 mRNA 和蛋白表达，减少了缺氧/复氧损伤细胞中一氧化氮的释放。特别是 50 mg/L 的三七总皂苷是最有效的剂量。这些发现表明，三七总皂苷能够以剂量依赖的方式减轻缺氧/复氧引起的神经元氧化应激损伤。
