The Graduate School of Nanoscience and Technology and ‡Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon, South Korea.
ACS Appl Mater Interfaces. 2014;6(21):18653-60. doi: 10.1021/am506873g. Epub 2014 Oct 31.
M13 bacteriophage (phage) was engineered for the use as a versatile template for preparing various nanostructured materials via genetic engineering coupled to enzymatic chemical conversions. First, we engineered the M13 phage to display TyrGluGluGlu (YEEE) on the pVIII coat protein and then enzymatically converted the Tyr residue to 3,4-dihydroxyl-l-phenylalanine (DOPA). The DOPA-displayed M13 phage could perform two functions: assembly and nucleation. The engineered phage assembles various noble metals, metal oxides, and semiconducting nanoparticles into one-dimensional arrays. Furthermore, the DOPA-displayed phage triggered the nucleation and growth of gold, silver, platinum, bimetallic cobalt-platinum, and bimetallic iron-platinum nanowires. This versatile phage template enables rapid preparation of phage-based prototype devices by eliminating the screening process, thus reducing effort and time.
M13 噬菌体（噬菌体）被设计为通过遗传工程与酶化学转化相结合，用于制备各种纳米结构材料的通用模板。首先，我们设计了 M13 噬菌体在 pVIII 外壳蛋白上展示 TyrGluGluGlu（YEEE），然后酶促将 Tyr 残基转化为 3,4-二羟基-l-苯丙氨酸（DOPA）。展示 DOPA 的 M13 噬菌体可以执行两个功能：组装和成核。工程噬菌体将各种贵金属、金属氧化物和半导体纳米颗粒组装成一维阵列。此外，展示 DOPA 的噬菌体触发了金、银、铂、双金属钴-铂和双金属铁-铂纳米线的成核和生长。这种多功能噬菌体模板通过消除筛选过程，从而减少工作量和时间，使基于噬菌体的原型器件的快速制备成为可能。
