Cho Eugene, Park Soon-Nang, Shin Yeseul, Lim Yun Kyong, Paek Jayoung, Kim Hak Kyun, Hwang Cheol Ho, Jo Eojin, Jin Dongchun, Chang Young-Hyo, Kook Joong-Ki
Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
Curr Microbiol. 2015 Feb;70(2):260-6. doi: 10.1007/s00284-014-0712-7. Epub 2014 Oct 16.
A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134(T), was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134(T) belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023(T) (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321(T) (93.8 %). The prevalent fatty acids of of strain ChDC B134(T) were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was D-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134(T) (=KCOM 1628(T) = KCTC 15227(T) = JCM 30223(T)) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.
从一名患者右上颌侧切牙与尖牙之间的术后感染病灶中分离出一株新型革兰氏阳性、厌氧、球菌状细菌，命名为ChDC B134(T)菌株，并通过多相分类分析对其进行了特征描述。16S rRNA基因序列分析表明，ChDC B134(T)菌株属于消化球菌属，因为它与吲哚消化球菌KCTC 15023(T)（94.0%）和不解糖消化球菌KCTC 3321(T)（93.8%）的序列相似。ChDC B134(T)菌株的主要脂肪酸为C16:0（20.3%）、C18:1顺式9（34.3%）和C18:0（13.2%）。DNA G+C含量为30.9 mol%。细胞壁二氨基酸为D-鸟氨酸，这是消化球菌属其他参考模式菌株共有的特性。基于表型、化学分类和系统发育分析结果，ChDC B134(T)菌株（=KCOM 1628(T)=KCTC 15227(T)=JCM 30223(T)）应被归类为消化球菌属一个新物种的模式菌株，为此提出了新种名米光消化球菌（Peptoniphilus mikwangii sp. nov.）。
