患有银屑病关节炎的患者肠道微生物多样性减少，其肠道微生物组与炎症性肠病中的失调类似。
Decreased bacterial diversity characterizes the altered gut microbiota in patients with psoriatic arthritis, resembling dysbiosis in inflammatory bowel disease.
机构信息
New York University and New York University Hospital for Joint Diseases, New York, New York.
出版信息
Arthritis Rheumatol. 2015 Jan;67(1):128-39. doi: 10.1002/art.38892.
OBJECTIVE
To characterize the diversity and taxonomic relative abundance of the gut microbiota in patients with never-treated, recent-onset psoriatic arthritis (PsA).
METHODS
High-throughput 16S ribosomal RNA pyrosequencing was utilized to compare the community composition of gut microbiota in patients with PsA (n = 16), patients with psoriasis of the skin (n = 15), and healthy, matched control subjects (n = 17). Samples were further assessed for the presence and levels of fecal and serum secretory IgA (sIgA), proinflammatory proteins, and fatty acids.
RESULTS
The gut microbiota observed in patients with PsA and patients with skin psoriasis was less diverse when compared to that in healthy controls. This could be attributed to the reduced presence of several taxa. Samples from both patient groups showed a relative decrease in abundance of Coprococcus species, while samples from PsA patients were also characterized by a significant reduction in Akkermansia, Ruminococcus, and Pseudobutyrivibrio. Supernatants of fecal samples from PsA patients revealed an increase in sIgA levels and decrease in RANKL levels. Analysis of fatty acids revealed low fecal quantities of hexanoate and heptanoate in both patients with PsA and patients with psoriasis.
CONCLUSION
Patients with PsA and patients with skin psoriasis had a lower relative abundance of multiple intestinal bacteria. Although some genera were concomitantly decreased in both conditions, PsA samples had a lower abundance of reportedly beneficial taxa. This gut microbiota profile in PsA was similar to that previously described in patients with inflammatory bowel disease and was associated with changes in specific inflammatory proteins unique to this group, and distinct from that in patients with skin psoriasis and healthy controls. Thus, the role of the gut microbiome in the continuum of psoriasis-PsA pathogenesis and the associated immune response merits further study.
目的
分析未经治疗、近期发病的银屑病关节炎（PsA）患者肠道微生物菌群的多样性和分类相对丰度。
方法
采用高通量 16S 核糖体 RNA 焦磷酸测序技术，比较了 16 例 PsA 患者、15 例皮肤银屑病患者和 17 例健康匹配对照者的肠道微生物菌群群落组成。进一步评估粪便和血清分泌型免疫球蛋白 A（sIgA）、促炎蛋白和脂肪酸的存在和水平。
结果
与健康对照者相比，PsA 患者和皮肤银屑病患者的肠道微生物菌群多样性降低，这可能归因于某些菌群的减少。两组患者样本中 Coprococcus 属的丰度相对降低，而 PsA 患者样本中 Akkermansia、Ruminococcus 和 Pseudobutyrivibrio 的丰度也显著降低。PsA 患者粪便样本上清液显示 sIgA 水平升高，RANKL 水平降低。脂肪酸分析显示，PsA 患者和皮肤银屑病患者粪便中 hexanoate 和 heptanoate 含量较低。
结论
PsA 患者和皮肤银屑病患者的多种肠道细菌相对丰度较低。虽然两种情况下一些属同时减少，但 PsA 样本中据称有益的类群丰度较低。这种 PsA 肠道微生物群谱与先前描述的炎症性肠病患者相似，与该组特有的特定炎症蛋白的变化相关，与皮肤银屑病患者和健康对照者不同。因此，肠道微生物组在银屑病-关节炎发病机制中的作用及其相关免疫反应值得进一步研究。
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