Jamshidi J, Movafagh A, Emamalizadeh B, Zare Bidoki A, Manafi A, Ghasemi Firouzabadi S, Shahidi G-A, Kazeminasab S, Petramfar P, Fazeli A, Motallebi M, Mortazavi-Tabatabaei S A, Kowsari A, Jafarian Z, Darvish H
Department of Biochemistry, Fasa University of Medical Sciences, Fasa, Iran.
Int J Immunogenet. 2014 Dec;41(6):508-11. doi: 10.1111/iji.12151. Epub 2014 Oct 15.
The rs3129882, a noncoding variant in HLA-DR, was found to be associated with Parkinson's disease (PD) using several genome-wide association studies. The aim of this replication study was to explore the relationship between this variant and PD in Iranian population. Genomic DNA was extracted from peripheral blood samples, and the rs3129882 SNP was genotyped using a PCR-RFLP method in 520 PD patients and 520 healthy Iranian controls. Significant differences were found in allele frequencies between patients and controls (χ(2) = 4.64, P = 0.031). Under additive and dominant models, the association of the SNP with PD risk is significant, where the A allele was observed to be protective. The results suggest that rs3129882 polymorphism may be a risk factor for PD in Iranian. This is the first study reporting such an association in this population. More replication studies are needed to confirm this data.
通过多项全基因组关联研究发现，位于人类白细胞抗原-DR（HLA-DR）区域的非编码变异rs3129882与帕金森病（PD）相关。本重复研究的目的是探讨该变异与伊朗人群中帕金森病的关系。从外周血样本中提取基因组DNA，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对520例帕金森病患者和520名健康伊朗对照者进行rs3129882单核苷酸多态性（SNP）基因分型。患者与对照者的等位基因频率存在显著差异（χ² = 4.64，P = 0.031）。在加性和显性模型下，该SNP与帕金森病风险的关联具有统计学意义，其中A等位基因被观察到具有保护作用。结果表明，rs3129882多态性可能是伊朗人群患帕金森病的一个风险因素。这是首次在该人群中报道此类关联的研究。需要更多的重复研究来证实这一数据。
