Branting C, Sund M L, Linder L E
Department of Oral Microbiology, Huddinge University Hospital, Karolinska Institute, Sweden.
Arch Oral Biol. 1989;34(5):347-53. doi: 10.1016/0003-9969(89)90108-8.
Adhesion of Candida albicans and Streptococcus mutans was studied by incubation of radiolabelled cells with acrylic test specimens in a chemically defined growth medium. Strep. mutans adhered firmly in the presence of sucrose, while C. albicans was only loosely attached to the acrylic in both glucose and sucrose media. Firm adhesion of C. albicans occurred when the yeast was incubated simultaneously with Strep. mutans, in the presence of sucrose. The adhesion of C. albicans was also stimulated by incubation with Strep. mutans culture supernatants. Adhesion was not affected by the presence of partially purified glucosyltransferase from Strep. mutans IB. Coaggregation between C. albicans and Strep. mutans upon growth in sucrose medium was observed by light and scanning electron microscopy. No coaggregation was observed in the presence of glucose.
通过在化学成分明确的生长培养基中，将放射性标记的细胞与丙烯酸测试标本一起孵育，研究了白色念珠菌和变形链球菌的黏附情况。在蔗糖存在的情况下，变形链球菌能牢固黏附，而在葡萄糖和蔗糖培养基中，白色念珠菌仅松散地附着于丙烯酸。当白色念珠菌与变形链球菌在蔗糖存在的情况下同时孵育时，白色念珠菌会发生牢固黏附。与变形链球菌培养上清液一起孵育也会刺激白色念珠菌的黏附。变形链球菌IB的部分纯化葡糖基转移酶的存在对黏附没有影响。通过光学显微镜和扫描电子显微镜观察到，在蔗糖培养基中生长时，白色念珠菌和变形链球菌之间会发生共聚。在葡萄糖存在的情况下未观察到共聚现象。
