Arzmi Mohd Hafiz, Dashper Stuart, Catmull Deanne, Cirillo Nicola, Reynolds Eric C, McCullough Michael
Oral Health CRC, Melbourne Dental School, The University of Melbourne, VIC 3053, Australia Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
Oral Health CRC, Melbourne Dental School, The University of Melbourne, VIC 3053, Australia.
FEMS Yeast Res. 2015 Aug;15(5):fov038. doi: 10.1093/femsyr/fov038. Epub 2015 Jun 7.
Microbial interactions are necessarily associated with the development of polymicrobial oral biofilms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strains were grown in RPMI-1640 and artificial saliva medium (ASM) whereas bacteria were grown in heart infusion broth. C. albicans, A. naeslundii and S. mutans were suspended to give 10(6), 10(7) and 10(8) cells mL(-1) respectively, in coaggregation buffer followed by a 1 h incubation. The absorbance difference at 620 nm (ΔAbs) between 0 h and 1 h was recorded. To study coaggregation, the same protocol was used, except combinations of microorganisms were incubated together. The mean ΔAbs% of autoaggregation of the majority of RPMI-1640-grown C. albicans was higher than in ASM grown. Coaggregation of C. albicans with A. naeslundii and/or S. mutans was variable among C. albicans strains. Scanning electron microscopy images showed that A. naeslundii and S. mutans coaggregated with C. albicans in dual- and triculture. In conclusion, the coaggregation of C. albicans, A. naeslundii and S. mutans is C. albicans strain dependent.
微生物相互作用必然与多微生物口腔生物膜的形成有关。本研究的目的是确定八株白色念珠菌与内氏放线菌和变形链球菌的共聚集情况。在自聚集试验中，白色念珠菌菌株在RPMI - 1640和人工唾液培养基（ASM）中培养，而细菌在心脏浸液肉汤中培养。将白色念珠菌、内氏放线菌和变形链球菌分别悬浮于共聚集缓冲液中，使其浓度分别达到10⁶、10⁷和10⁸个细胞/mL⁻¹，然后孵育1小时。记录0小时和1小时时620nm处的吸光度差值（ΔAbs）。为了研究共聚集，采用相同的方案，只是将微生物组合在一起孵育。大多数在RPMI - 1640中培养的白色念珠菌的自聚集平均ΔAbs%高于在ASM中培养的。白色念珠菌与内氏放线菌和/或变形链球菌的共聚集在白色念珠菌菌株之间存在差异。扫描电子显微镜图像显示，在内氏放线菌和变形链球菌与白色念珠菌的二元和三元培养中存在共聚集现象。总之，白色念珠菌、内氏放线菌和变形链球菌的共聚集依赖于白色念珠菌菌株。
