Laha R, Bhattacharjee K, Sarmah P C, Das M, Goswami A, Sarma D, Sen A
Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
Department of Parasitology, College of Veterinary Science, Khanapara, Guwahati, Assam 781022 India.
J Parasit Dis. 2014 Dec;38(4):389-93. doi: 10.1007/s12639-013-0261-1. Epub 2013 Feb 16.
The objective of the study was to detect Babesia infections in pet dogs of a north-eastern state of India. The diagnostic efficacy of Babesia infection by polymerase chain reaction (PCR) technique has been compared with microscopy examination. For this, a total of 111 blood samples of pet dogs presented at clinical complex of the College of Veterinary Science, Guwahati, Assam with clinical signs suspected for Babesia infection subjected to the study. A total of 44 (39.63 %) dogs were diagnosed as positive for Babesia infections after microscopic examination. Among these, Babesia canis infection was diagnosed in 5 dogs (4.50 %) and B. gibsoni infection in 39 (35.13 %) dogs microscopically in Giemsa stained blood smears. Molecular diagnosis using PCR detected 63 (56.75 %) dogs positive for Babesia infection. Single infection with B. canis was found in 9 (8.10 %) dogs while B. gibsoni alone was detected in 3 (2.70 %) dogs. Mixed infections by both these species were detected in 51 (45.94 %) dogs. Overall, PCR detected 54 (48.64 %) dogs as B. gibsoni and 60 (54.05 %) dogs as B. canis positive.
该研究的目的是检测印度东北部一个邦的宠物狗中的巴贝斯虫感染情况。已将聚合酶链反应（PCR）技术诊断巴贝斯虫感染的效果与显微镜检查进行了比较。为此，对总共111份呈现出疑似巴贝斯虫感染临床症状的宠物狗血样进行了研究，这些血样来自阿萨姆邦古瓦哈蒂兽医科学学院的临床综合机构。经显微镜检查，共有44只（39.63%）狗被诊断为巴贝斯虫感染阳性。其中，在吉姆萨染色血涂片显微镜检查中，5只（4.50%）狗被诊断为犬巴贝斯虫感染，39只（35.13%）狗被诊断为吉氏巴贝斯虫感染。使用PCR进行分子诊断检测出63只（56.75%）狗为巴贝斯虫感染阳性。9只（8.10%）狗被发现单一感染犬巴贝斯虫，3只（2.70%）狗仅检测出吉氏巴贝斯虫。51只（45.94%）狗检测出这两种巴贝斯虫的混合感染。总体而言，PCR检测出54只（48.64%）狗为吉氏巴贝斯虫阳性，60只（54.05%）狗为犬巴贝斯虫阳性。
