Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Breast Cancer. 2014 Sep;17(3):219-25. doi: 10.4048/jbc.2014.17.3.219. Epub 2014 Sep 30.
Breast cancer is the most common type of cancer in women. Despite various pharmacological developments, the identification of new therapies is still required for treating breast cancer. Crab is often recommended as a traditional medicine for cancer. This study aimed to determine the in vitro effect of a hydroalcoholic crab shell extract on a breast cancer cell line.
In this experimental study, MCF7 breast cancer cell line was used. Crab shell was powdered and a hydroalcoholic (70° ethanol) extract was prepared. Five concentrations (100, 200, 400, 800, and 1,000 µg/mL) were added to the cells for three periods, 24, 48, and 72 hours. The viability of the cells were evaluated using trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Cell apoptosis was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling method. Nitric oxide (NO) level was assessed using the Griess method. Data were analyzed using analysis of variance, and p<0.05 was considered significant.
Cell viability decreased depending on dose and time, and was significantly different in the groups that were treated with 400, 800, and 1,000 µg/mL doses compared to that in the control group (p<0.001). Increasing the dose significantly increased apoptosis (p<0.001). NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050).
The crab shell extract inhibited the proliferation of MCF7 cells by increasing apoptosis and decreasing NO production.
乳腺癌是女性最常见的癌症类型。尽管有各种药物学的发展，但仍需要寻找新的疗法来治疗乳腺癌。螃蟹常被推荐为一种治疗癌症的传统药物。本研究旨在确定一种螃蟹壳水醇提取物对乳腺癌细胞系的体外作用。
在这项实验研究中，使用 MCF7 乳腺癌细胞系。将螃蟹壳粉碎并制备水醇（70°乙醇）提取物。将五个浓度（100、200、400、800 和 1000μg/mL）添加到细胞中，分别作用 24、48 和 72 小时。使用台盼蓝和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估细胞活力。使用末端脱氧核苷酸转移酶 dUTP 缺口末端标记法测定细胞凋亡。使用格里斯法评估一氧化氮（NO）水平。使用方差分析分析数据，p<0.05 被认为具有统计学意义。
细胞活力随剂量和时间的变化而降低，与对照组相比，用 400、800 和 1000μg/mL 剂量处理的组中细胞活力明显降低（p<0.001）。增加剂量可显著增加细胞凋亡（p<0.001）。随着提取物浓度的增加，MCF7 细胞的 NO 分泌呈剂量和时间依赖性显著降低（p<0.050）。
螃蟹壳提取物通过增加细胞凋亡和减少 NO 生成来抑制 MCF7 细胞的增殖。
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