间苯三酚减轻自由基诱导的氧化应激。
Phloroglucinol Attenuates Free Radical-induced Oxidative Stress.
作者信息
So Mi Jung, Cho Eun Ju
机构信息
Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University, Busan 609-735, Korea.
出版信息
Prev Nutr Food Sci. 2014 Sep;19(3):129-35. doi: 10.3746/pnf.2014.19.3.129.
The protective role of phloroglucinol against oxidative stress and stress-induced premature senescence (SIPS) was investigated in vitro and in cell culture. Phloroglucinol had strong and concentration-dependent radical scavenging effects against nitric oxide (NO), superoxide anions (O2 (-)), and hydroxyl radicals. In this study, free radical generators were used to induce oxidative stress in LLC-PK1 renal epithelial cells. Treatment with phloroglucinol attenuated the oxidative stress induced by peroxyl radicals, NO, O2 (-), and peroxynitrite. Phloroglucinol also increased cell viability and decreased lipid peroxidation in a concentration-dependent manner. WI-38 human diploid fibroblast cells were used to investigate the protective effect of phloroglucinol against hydrogen peroxide (H2O2)-induced SIPS. Phloroglucinol treatment attenuated H2O2-induced SIPS by increasing cell viability and inhibited lipid peroxidation, suggesting that treatment with phloroglucinol should delay the aging process. The present study supports the promising role of phloroglucinol as an antioxidative agent against free radical-induced oxidative stress and SIPS.
在体外和细胞培养中研究了间苯三酚对氧化应激和应激诱导的早衰（SIPS）的保护作用。间苯三酚对一氧化氮（NO）、超氧阴离子（O2(-)）和羟自由基具有强大的、浓度依赖性的自由基清除作用。在本研究中，使用自由基发生器在LLC-PK1肾上皮细胞中诱导氧化应激。用间苯三酚处理可减轻过氧自由基、NO、O2(-)和过氧亚硝酸根诱导的氧化应激。间苯三酚还以浓度依赖性方式增加细胞活力并降低脂质过氧化。使用WI-38人二倍体成纤维细胞研究间苯三酚对过氧化氢（H2O2）诱导的SIPS的保护作用。间苯三酚处理通过增加细胞活力减轻H2O2诱导的SIPS并抑制脂质过氧化，表明用间苯三酚处理应能延缓衰老过程。本研究支持间苯三酚作为抗自由基诱导的氧化应激和SIPS的抗氧化剂的潜在作用。
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