Sullivan Shane Z, Muir Ryan D, Newman Justin A, Carlsen Mark S, Sreehari Suhas, Doerge Chris, Begue Nathan J, Everly R Michael, Bouman Charles A, Simpson Garth J
Opt Express. 2014 Oct 6;22(20):24224-34. doi: 10.1364/OE.22.024224.
A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Since sub-trajectory and full-trajectory imaging are simply different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired. The optical hardware required to perform Lissajous imaging represents only a minor modification to established beam-scanning hardware, combined with additional control and data acquisition electronics. Preliminary studies based on laser transmittance imaging and polarization-dependent second harmonic generation microscopy support the viability of the approach both for detection of subtle changes in large signals and for trace-light detection of transient fluctuations.
描述了一种基于李萨如轨迹成像的简单光束扫描光学设计，用于在多个同步数据采集通道上实现高达千赫兹帧率的光学成像。简而言之，两个快速扫描共振镜将光束引导在一条迂回的轨迹上穿过视场，轨迹重复时间由镜子周期的最小公倍数给出。将原始时域数据切割成子轨迹，并结合基于模型的图像重建（MBIR）三维内插算法，可实现比李萨如轨迹重复时间高得多的有效帧率。由于子轨迹成像和全轨迹成像只是分析同一数据的不同方法，因此可以同时获取分辨率相对较低的高帧率图像和分辨率较高的低帧率图像。执行李萨如成像所需的光学硬件只是对现有光束扫描硬件进行了微小修改，并结合了额外的控制和数据采集电子设备。基于激光透射率成像和偏振相关二次谐波产生显微镜的初步研究支持了该方法在检测大信号中的细微变化以及检测瞬态波动的痕量光方面的可行性。
