Qi Can, Ma Hui, Zhang Hong-Tuan, Gao Jing-Da, Xu Yong
Department of Urology, the Second Hospital of Tianjin Medical University, National Key Clinical Specialty of Urology, Tianjin Key Institute of Urology, Tianjin, China.
Histopathology. 2015 Apr;66(5):650-7. doi: 10.1111/his.12587. Epub 2014 Dec 22.
Nucleobindin 2 (NUCB2) has been reported to play an important role in both tumorigenesis and cancer progression. This study aimed to examine the clinical significance of NUCB2 expression in clear cell renal cell carcinoma (ccRCC).
The expression level of NUCB2 and its correlation with clinicopathological parameters was analysed in 188 ccRCC tissues and adjacent non-cancerous tissues by immunohistochemistry. Samples from eight ccRCC patients were examined by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the correlation between NUCB2 expression and the prognosis of ccRCC patients. The expression level of NUCB2 was found to be significantly higher in ccRCC tumours compared to adjacent non-cancerous tissues using immunohistochemistry, Western blotting and qRT-PCR. Moreover, high NUCB2 tumour expression was associated with high T stage and metastasis and shorter overall survival. Univariate and multivariate analysis confirmed that NUCB2 was an independent prognostic factor for overall survival.
Our results show that NUCB2 plays an important role in tumorigenesis and progression and is a potential molecular biomarker for the diagnosis and targeted therapy of ccRCC.
据报道，核结合蛋白2（NUCB2）在肿瘤发生和癌症进展中均发挥重要作用。本研究旨在探讨NUCB2表达在透明细胞肾细胞癌（ccRCC）中的临床意义。
采用免疫组织化学方法分析188例ccRCC组织及癌旁非癌组织中NUCB2的表达水平及其与临床病理参数的相关性。采用蛋白质免疫印迹法和定量实时聚合酶链反应（qRT-PCR）检测8例ccRCC患者的样本。采用Kaplan-Meier分析和Cox比例风险回归模型研究NUCB2表达与ccRCC患者预后的相关性。通过免疫组织化学、蛋白质免疫印迹法和qRT-PCR发现，与癌旁非癌组织相比，ccRCC肿瘤中NUCB2表达水平显著更高。此外，高NUCB2肿瘤表达与高T分期、转移及较短的总生存期相关。单因素和多因素分析证实，NUCB2是总生存期的独立预后因素。
我们的结果表明，NUCB2在肿瘤发生和进展中起重要作用，是ccRCC诊断和靶向治疗的潜在分子生物标志物。
