整合宿主因子（IHF）对噬菌体Mu阻遏物转录的调控取决于早期转录的水平。
Regulation of phage Mu repressor transcription by IHF depends on the level of the early transcription.
作者信息
van Rijn P A, Goosen N, Turk S C, van de Putte P
机构信息
Department of Biochemistry, Gorlaeus Laboratoria, Leiden University, The Netherlands.
出版信息
Nucleic Acids Res. 1989 Dec 25;17(24):10203-12. doi: 10.1093/nar/17.24.10203.
Abstract
Integration Host Factor (IHF) of E. coli can stimulate both early and repressor transcription of bacteriophage Mu. We introduced several mutations in the early promoter (Pe) and studied the effect of these mutations on the stimulation of early and repressor transcription by IHF. All mutant promoters are still positive regulated by IHF, but the level of stimulation is dependent on the strength of the promoter. The strength of the early promoter has an even greater impact on the regulation of the repressor promoter by IHF: stimulation is observed in the presence of a relatively weak Pe, whereas with a strong Pe the repressor promoter Pc is inhibited by IHF. This inhibition is most probably due to an interference of the early transcription with the opposing repressor transcription. The implication of this type of regulation for the Mu life cycle is discussed.
摘要
大肠杆菌的整合宿主因子（IHF）可刺激噬菌体Mu的早期转录和阻遏物转录。我们在早期启动子（Pe）中引入了几个突变，并研究了这些突变对IHF刺激早期转录和阻遏物转录的影响。所有突变启动子仍受IHF的正调控，但刺激水平取决于启动子的强度。早期启动子的强度对IHF对阻遏物启动子的调控影响更大：在相对较弱的Pe存在时观察到刺激作用，而在强Pe存在时，阻遏物启动子Pc受到IHF的抑制。这种抑制很可能是由于早期转录对相反的阻遏物转录的干扰所致。本文讨论了这种调控类型对Mu生命周期的影响。
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