Goosen N, van Heuvel M, Moolenaar G F, van de Putte P
Gene. 1984 Dec;32(3):419-26. doi: 10.1016/0378-1119(84)90017-9.
Two leftward Pc promoters for the repressor gene of bacteriophage Mu have been localized by fusions of the promoter region to the structural galK gene and by S1 nuclease mapping. Transcription initiated at the left-end-proximal promoter (Pc-1) starts 23 bp ahead of the c gene. The second promoter (Pc-2) is located 200 bp from the translation start codon of gene c. The RNA initiated from Pc-2 overlaps 35 bp with the rightward transcript from the early Mu promoter (Pe). The expression from Pe and both repressor promoters is positively regulated by the Escherichia coli HimD (Hip) protein, probably acting as a subunit of the integration host factor (IHF). Two overlapping sequences matching the consensus for the IHF binding site (ihf) are found between Pe and Pc-1.
通过将噬菌体Mu阻遏基因的启动子区域与结构基因galK融合以及S1核酸酶图谱分析，已定位出两个向左的Pc启动子。从左端近端启动子（Pc - 1）起始的转录在c基因之前23个碱基对处开始。第二个启动子（Pc - 2）位于距基因c的翻译起始密码子200个碱基对处。从Pc - 2起始的RNA与早期Mu启动子（Pe）的向右转录本重叠35个碱基对。Pe以及两个阻遏启动子的表达受到大肠杆菌HimD（Hip）蛋白的正调控，该蛋白可能作为整合宿主因子（IHF）的一个亚基发挥作用。在Pe和Pc - 1之间发现了两个与IHF结合位点（ihf）共有序列匹配的重叠序列。
