大肠杆菌的整合宿主因子通过两种不同机制调控噬菌体Mu的早期转录和阻遏物转录。
Integration host factor of Escherichia coli regulates early- and repressor transcription of bacteriophage Mu by two different mechanisms.
作者信息
van Rijn P A, Goosen N, van de Putte P
机构信息
Department of Molecular Genetics, State University of Leiden, The Netherlands.
出版信息
Nucleic Acids Res. 1988 May 25;16(10):4595-605. doi: 10.1093/nar/16.10.4595.
Abstract
Integration host factor (IHF) of E. coli positively regulates both early and repressor transcription of bacteriophage Mu. In this paper we show that although binding of IHF to the same binding site is responsible for both types of transcription regulation, the mechanisms by which these regulations occur are different: Activation of transcription from the early promoter (Pe) requires a helix-dependent orientation of IHF- and RNA polymerase binding sites on the DNA helix with a limited distance between both sites. Activation of repressor transcription shows no helix dependency between promoter and IHF binding site and the distance between both sites can be enlarged at least by 100 base pairs without affecting the positive control. A possible mechanism for both types of transcription stimulation will be discussed.
摘要
大肠杆菌的整合宿主因子（IHF）对噬菌体Mu的早期转录和阻遏物转录均具有正向调节作用。在本文中，我们表明，尽管IHF与相同结合位点的结合负责这两种转录调节类型，但这些调节发生的机制不同：早期启动子（Pe）转录的激活需要IHF和RNA聚合酶结合位点在DNA螺旋上呈螺旋依赖性定向，且两个位点之间的距离有限。阻遏物转录的激活在启动子和IHF结合位点之间未显示螺旋依赖性，并且两个位点之间的距离至少可以扩大100个碱基对而不影响正向调控。将讨论这两种转录刺激类型的可能机制。
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Primary structure of the himA gene of Escherichia coli: homology with DNA-binding protein HU and association with the phenylalanyl-tRNA synthetase operon.大肠杆菌himA基因的一级结构：与DNA结合蛋白HU的同源性以及与苯丙氨酰-tRNA合成酶操纵子的关联。
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