Deconinck Eric, Custers Deborah, De Beer Jacques Omer
Division of Food, Medicines and Consumer Safety, Section Medicinal Products, Scientific Institute of Public Health (WIV-ISP), Rue Juliette Wytsmanstraat 14, Brussels, 1050, Belgium,
Methods Mol Biol. 2015;1208:181-99. doi: 10.1007/978-1-4939-1441-8_14.
The standard procedures for the identification, authentication, and quality control of medicinal plants and herbs are nowadays limited to pure herbal products. No guidelines or procedures, describing the detection or identification of a targeted plant or herb in pharmaceutical preparations or dietary supplements, can be found. In these products the targeted plant is often present together with other components of herbal or synthetic origin. This chapter describes a strategy for the fast development of a chromatographic fingerprint approach that allows the identification of a targeted plant in herbal preparations and dietary supplements. The strategy consists of a standard chromatographic gradient that is tested for the targeted plant with different extraction solvents and different mobile phases. From the results obtained, the optimal fingerprint is selected. Subsequently the samples are analyzed according to the selected methodological parameters, and the obtained fingerprints can be compared with the one obtained for the pure herbal product or a standard preparation. Calculation of the dissimilarity between these fingerprints will result in a probability of presence of the targeted plant. Optionally mass spectrometry can be used to improve specificity, to confirm identification, or to identify molecules with a potential medicinal or antioxidant activity.
目前，药用植物和草药的鉴定、认证及质量控制标准程序仅限于纯草药产品。在药物制剂或膳食补充剂中，尚未发现有关检测或鉴定目标植物或草药的指南或程序。在这些产品中，目标植物通常与其他草药或合成来源的成分共存。本章描述了一种快速开发色谱指纹图谱方法的策略，该方法可用于鉴定草药制剂和膳食补充剂中的目标植物。该策略包括一个标准色谱梯度，用不同的提取溶剂和不同的流动相对目标植物进行测试。根据所得结果，选择最佳指纹图谱。随后，根据选定的方法参数对样品进行分析，并将所得指纹图谱与纯草药产品或标准制剂的指纹图谱进行比较。计算这些指纹图谱之间的差异将得出目标植物存在的概率。可选择使用质谱法来提高特异性、确认鉴定结果或鉴定具有潜在药用或抗氧化活性的分子。
