Reiser H, Benacerraf B
Department of Pathology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA.
Proc Natl Acad Sci U S A. 1989 Dec;86(24):10069-73. doi: 10.1073/pnas.86.24.10069.
We have analyzed the requirements of highly purified, resting murine CD4+ T lymphocytes for activation mediated by the lectin Con A and by monoclonal antibodies against the CD3 and Thy-1 molecules. Our results indicate that both the Ia-positive B-lymphoblastoid cell line M12 and its Ia-negative variant M12.C3 can provide the costimulatory activity necessary for these activation pathways. The costimulatory function is preserved upon fixation with paraformaldehyde, indicating that the costimulatory molecule(s) is (are) constitutively expressed on the cell surface. Our experiments also point to interesting differences between the M12 cell line and syngeneic Ia-positive antigen-presenting cells in generating a syngeneic mixed lymphocyte reaction. Finally, we show that the CD4+ T cell-M12.C3 cell interaction can be used to screen for interesting monoclonal antibodies that affect cell function.
我们分析了高纯度、静止的小鼠CD4+ T淋巴细胞对于由凝集素刀豆蛋白A以及针对CD3和Thy-1分子的单克隆抗体介导的激活的需求。我们的结果表明，Ia阳性的B淋巴母细胞系M12及其Ia阴性变体M12.C3均可提供这些激活途径所需的共刺激活性。用多聚甲醛固定后，共刺激功能得以保留，这表明共刺激分子在细胞表面组成性表达。我们的实验还指出了M12细胞系与同基因Ia阳性抗原呈递细胞在产生同基因混合淋巴细胞反应方面存在有趣的差异。最后，我们表明CD4+ T细胞与M12.C3细胞的相互作用可用于筛选影响细胞功能的有趣单克隆抗体。
