斑马鱼作为研究活体黏液生理学的模型。
Zebrafish as a model to study live mucus physiology.
作者信息
Jevtov Irena, Samuelsson Tore, Yao Grace, Amsterdam Adam, Ribbeck Katharina
机构信息
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA.
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden.
出版信息
Sci Rep. 2014 Oct 17;4:6653. doi: 10.1038/srep06653.
Dysfunctional mucus barriers can result in important pulmonary and gastrointestinal conditions, but model systems to study the underlying causes are largely missing. We identified and characterized five mucin homologues in zebrafish, and demonstrated a strategy for fluorescence labeling of one selected mucin. These tools can be used for in vivo experiments and in pharmacological and genetic screens to study the dynamics and mechanisms of mucosal physiology.
功能失调的黏液屏障可导致重要的肺部和胃肠道疾病，但用于研究其潜在病因的模型系统基本缺失。我们在斑马鱼中鉴定并表征了五种黏蛋白同源物，并展示了一种对选定的一种黏蛋白进行荧光标记的策略。这些工具可用于体内实验以及药理和基因筛选，以研究黏膜生理学的动态变化和机制。
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