van Wachem P B, Schakenraad J M, Feijen J, Beugeling T, van Aken W G, Blaauw E H, Nieuwenhuis P, Molenaar I
Department of Chemical Engineering, Twente University, Enschede, The Netherlands.
Biomaterials. 1989 Oct;10(8):532-9. doi: 10.1016/0142-9612(89)90058-6.
The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture poly(ethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than those found on PETP. When cells were seeded in the presence of serum, extensive cell spreading on both PETP and TCPETP was observed after the first 30 min. Thereafter, spread cells appeared to withdraw from the PETP surface, resulting in irregularly shaped cells. Complete cell spreading occurred on TCPETP. Complete cell spreading also occurred on PETP and TCPETP when HEC had first been seeded from phosphate buffer solution and serum was supplied after 30 min. Furthermore, HEC spread on both PETP and TCPETP when the surfaces were precoated with protein(s), which promotes cell adhesion. However, when plasma was used for the coating, spread cells did not proliferate in a monolayer pattern. This study shows that TCPETP is, in general, a better surface for adhesion and proliferation of HEC than is PETP, suggesting that vascular prostheses with a TCPETP-like surface will perform better in vivo than prostheses made of PETP.
采用光学显微镜和电子显微镜研究了人内皮细胞（HEC）在疏水性聚对苯二甲酸乙二酯（PETP）和适度可湿性组织培养聚对苯二甲酸乙二酯（TCPETP）上的体外黏附与铺展情况。黏附在TCPETP上的HEC数量总是高于在PETP上的数量。当细胞在血清存在的情况下接种时，最初30分钟后在PETP和TCPETP上均观察到细胞广泛铺展。此后，铺展的细胞似乎从PETP表面缩回，导致细胞形状不规则。在TCPETP上发生了完全的细胞铺展。当HEC首先从磷酸盐缓冲溶液中接种并在30分钟后加入血清时，在PETP和TCPETP上也发生了完全的细胞铺展。此外，当表面预先用促进细胞黏附的蛋白质包被时，HEC在PETP和TCPETP上均能铺展。然而，当用血浆进行包被时，铺展的细胞不会以单层模式增殖。本研究表明，总体而言，TCPETP比PETP更适合HEC的黏附与增殖，这表明具有类似TCPETP表面的血管假体在体内的性能将优于由PETP制成的假体。
