Morales-García M Rosario, García-Méndez Nayely, Regalado-Jacobo S Diana, López-Merino Anidé, Contreras-Rodríguez Araceli
Rev Chilena Infectol. 2014 Aug;31(4):425-33. doi: 10.4067/S0716-10182014000400008.
Human brucellosis diagnosis is based on isolation of Brucella spp. from blood or tissue cultures with a positivity rate of 40-70% and serology techniques are used as complementary tools; recently molecular biology diagnostic techniques have been developed intending to optimize the etiological confirmation.
The main objective of this work was to compare the polymerase chain reaction (PCR), against serological diagnostic tests during the clinical follow-up of a family presenting brucellosis.
Seven family members who lived in the urban area of Mexico City, were monitored using the Rose Bengal test, the agglutination test as well as agglutination with 2 mecapto ethanol, blood cultures and serum PCR for a period of 27 months. The suspected source of infection was fresh goat cheese from a known endemic zone.
Brucella melitensis was isolated from the blood cultures of two patients. All of the patients were positive in serological and PCR tests at the beginning of this follow-up. At the end of the study, three patients responded well to the treatment and showed negative results in the serological and PCR tests. While two patients with diabetes mellitus type 2, showed positive results in the serological and PCR tests as well as persistent symptoms.
Clinical follow-up of patients with brucellosis is of great importance, to properly evaluate the given treatment. In this sense the PCR is a great supporting tool in diagnostic testing.
人类布鲁氏菌病的诊断基于从血液或组织培养物中分离出布鲁氏菌属，阳性率为40%-70%，血清学技术用作辅助工具；最近已开发出分子生物学诊断技术以优化病因学确认。
这项工作的主要目的是在一个患布鲁氏菌病家庭的临床随访期间，将聚合酶链反应（PCR）与血清学诊断测试进行比较。
对居住在墨西哥城城区的七名家庭成员进行了为期27个月的监测，采用了玫瑰红试验、凝集试验以及2-巯基乙醇凝集试验、血培养和血清PCR。疑似感染源是来自一个已知疫区的新鲜山羊奶酪。
从两名患者的血培养物中分离出羊种布鲁氏菌。在随访开始时，所有患者的血清学和PCR检测均呈阳性。在研究结束时，三名患者对治疗反应良好，血清学和PCR检测结果均为阴性。而两名2型糖尿病患者的血清学和PCR检测结果呈阳性，且症状持续。
对布鲁氏菌病患者进行临床随访对于正确评估所给予的治疗非常重要。从这个意义上说，PCR是诊断测试中的一个很好的辅助工具。
