纸条萃取结合液相色谱-串联质谱法在药代动力学中的应用
Application of Paper Strip Extraction in Combination with LC-MS-MS in Pharmacokinetics.
作者信息
Legette LeeCole L, Reed Ralph L, Murty Lia, Maier Claudia S, Stevens Jan F
机构信息
Linus Pauling Institute and the College of Pharmacy at Oregon State University in Corvallis, Oregon.
College of Pharmacy at Oregon State University.
出版信息
Spectroscopy (Springf). 2013 Oct 1;39(10):s18-s25.
Abstract
Recent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation. In particular, the dried blood spot (DBS) sampling technique has numerous advantages compared to traditional methods such as liquid-liquid extraction, including the use of small sample volumes, less sample processing, and less exposure to toxic solvents (ether, methyl -butyl ether [MTBE], and dichloromethane). In this article, we discuss the adaptation of DBS technology to develop and validate a novel paper strip extraction method for the analysis of natural product metabolites in biological samples obtained from a human pharmacokinetic study of xanthohumol, a hop prenylflavonoid.
摘要
制药行业采样技术的最新进展引发了人们对改进萃取方法以实现更高效分析物检测和定量的浓厚兴趣。特别是，与液液萃取等传统方法相比，干血斑（DBS）采样技术具有诸多优势，包括使用的样本体积小、样本处理量少以及接触有毒溶剂（乙醚、甲基叔丁基醚[MTBE]和二氯甲烷）的机会少。在本文中，我们讨论了如何采用DBS技术来开发和验证一种新型纸条萃取方法，用于分析从人药代动力学研究中获得的生物样本中的天然产物代谢物，该研究针对的是一种啤酒花异戊烯基黄酮——黄腐酚。
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本文引用的文献
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Method development and validation for ultra-high-pressure LC/MS/MS determination of hop prenylflavonoids in human serum.超高压液相色谱/串联质谱法测定人血清中啤酒花异戊烯基黄酮的方法开发与验证
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Pharmacokinetics of xanthohumol and metabolites in rats after oral and intravenous administration.黄腐酚及其代谢物在大鼠口服和静脉注射后的药代动力学。
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