Okocha Ce, Manafa Po, Ozomba Jo, Ulasi To, Chukwuma Go, Aneke Jc
Department of Hematology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Anambra State, Nigeria.
Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University Nnewi Campus, Nnewi, Anambra State, Nigeria.
Ann Med Health Sci Res. 2014 Sep;4(5):701-5. doi: 10.4103/2141-9248.141523.
Evidence suggests that sickle cell disease (SCD) is associated with a chronic inflammatory state. C-reactive protein (CRP) is known to modulate inflammation. Its role in the chronic inflammation of SCD may make it valuable as a therapeutic target.
The aim was to determine CRP levels in SCD subjects in asymptomatic steady state (ASS) and crisis and correlate these with severity scores in the ASS.
We measured the level of CRP in 30 hemoglobin SS (HbSS) individuals in ASS and seven in crisis. As controls, we measured CRP in 50 individuals each who were hemoglobin AS and hemoglobin AA respectively, using enzyme linked immunoabsorbent assay based commercially available kits from East Wing Diagnostic Limited Full blood count (white blood cell [WBC]) was done for the ASS HbSS individuals using a cell counter and their disease severity calculated by an objective scoring method.
Our results showed that ASS HbSS individuals had significantly higher CRP levels compared with the controls. The HbSS individuals in crisis also had a significantly higher level of CRP compared to the ASS HbSS individuals. Disease severity and WBC were found to be negatively correlated with CRP levels (P = 0.17; and 0.73, respectively).
Our results suggest that increased levels of CRP in ASS HbSS individuals may play a protective role in SCD leading to better disease outcome, and may have value as a therapeutic target.
有证据表明镰状细胞病（SCD）与慢性炎症状态相关。已知C反应蛋白（CRP）可调节炎症。其在SCD慢性炎症中的作用可能使其成为有价值的治疗靶点。
目的是确定处于无症状稳定状态（ASS）和危机期的SCD受试者的CRP水平，并将这些水平与ASS期的严重程度评分相关联。
我们测量了30名处于ASS期的血红蛋白SS（HbSS）个体和7名处于危机期个体的CRP水平。作为对照，我们分别使用East Wing Diagnostic Limited公司基于酶联免疫吸附测定的市售试剂盒，测量了50名血红蛋白AS个体和50名血红蛋白AA个体的CRP水平。使用血细胞计数器对ASS期HbSS个体进行全血细胞计数（白细胞[WBC]），并通过客观评分方法计算其疾病严重程度。
我们的结果显示，与对照组相比，ASS期HbSS个体的CRP水平显著更高。与ASS期HbSS个体相比，处于危机期的HbSS个体的CRP水平也显著更高。发现疾病严重程度和WBC与CRP水平呈负相关（P值分别为0.17和0.73）。
我们的结果表明，ASS期HbSS个体中CRP水平升高可能在SCD中发挥保护作用，从而导致更好的疾病结局，并且可能具有作为治疗靶点的价值。
