糖皮质激素对商陆丝裂原刺激的人淋巴细胞免疫球蛋白合成的增强作用。
Glucocorticosteroid enhancement of immunoglobulin synthesis by pokeweed mitogen-stimulated human lymphocytes.
作者信息
Cooper D A, Duckett M, Hansen P, Petts V, Penny R
出版信息
Clin Exp Immunol. 1981 Apr;44(1):129-36.
We studied the role of the T lymphocyte in GCS enhancement of PWM-stimulated IgG synthesis by human peripheral blood mononuclear cells. Purified T or B lymphocyte subpopulations were pretreated with 10(-6) M prednisolone or recombined at various T:B ratios and 10(-6) M prednisolone was added. PWM-stimulated IgG synthesis was measured in the culture supernatants at 8 days by radioimmunoassay. Addition of prednisolone to cultures of autologous and allogeneic reconstituted mixtures of T and B lymphocytes resulted in enhancement of PWM-stimulated IgG synthesis. This effect was observed with constant and increasing numbers of lymphocytes in culture, independent of T:B ratio and occurred with purified B lymphocytes containing monocytes. Pretreatment of purified B lymphocytes containing monocytes but not purified T lymphocytes with prednisolone enhanced PWM-stimulated IgG synthesis in reconstituted mixtures of T and B lymphocytes. We propose that GCS enhancement of PWM-stimulated IgG synthesis by human mononuclear cells is independent of T lymphocyte regulation.
我们研究了T淋巴细胞在糖皮质激素（GCS）增强人外周血单个核细胞经美洲商陆有丝分裂原（PWM）刺激的IgG合成中的作用。纯化的T或B淋巴细胞亚群用10⁻⁶ M泼尼松龙预处理，或以不同的T:B比例重组并加入10⁻⁶ M泼尼松龙。在第8天通过放射免疫测定法测量培养上清液中PWM刺激的IgG合成。向自体和同种异体重建的T和B淋巴细胞混合物培养物中添加泼尼松龙导致PWM刺激的IgG合成增强。在培养中淋巴细胞数量恒定和增加的情况下均观察到这种效应，与T:B比例无关，并且在含有单核细胞的纯化B淋巴细胞中也会出现。用泼尼松龙预处理含有单核细胞的纯化B淋巴细胞而非纯化T淋巴细胞，可增强T和B淋巴细胞重建混合物中PWM刺激的IgG合成。我们提出，GCS增强人单核细胞经PWM刺激的IgG合成与T淋巴细胞调节无关。
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