Cooper D A, Duckett M, Hansen P, Petts V, Penny R
Clin Exp Immunol. 1981 Apr;44(1):129-36.
We studied the role of the T lymphocyte in GCS enhancement of PWM-stimulated IgG synthesis by human peripheral blood mononuclear cells. Purified T or B lymphocyte subpopulations were pretreated with 10(-6) M prednisolone or recombined at various T:B ratios and 10(-6) M prednisolone was added. PWM-stimulated IgG synthesis was measured in the culture supernatants at 8 days by radioimmunoassay. Addition of prednisolone to cultures of autologous and allogeneic reconstituted mixtures of T and B lymphocytes resulted in enhancement of PWM-stimulated IgG synthesis. This effect was observed with constant and increasing numbers of lymphocytes in culture, independent of T:B ratio and occurred with purified B lymphocytes containing monocytes. Pretreatment of purified B lymphocytes containing monocytes but not purified T lymphocytes with prednisolone enhanced PWM-stimulated IgG synthesis in reconstituted mixtures of T and B lymphocytes. We propose that GCS enhancement of PWM-stimulated IgG synthesis by human mononuclear cells is independent of T lymphocyte regulation.
我们研究了T淋巴细胞在糖皮质激素（GCS）增强人外周血单个核细胞经美洲商陆有丝分裂原（PWM）刺激的IgG合成中的作用。纯化的T或B淋巴细胞亚群用10⁻⁶ M泼尼松龙预处理，或以不同的T:B比例重组并加入10⁻⁶ M泼尼松龙。在第8天通过放射免疫测定法测量培养上清液中PWM刺激的IgG合成。向自体和同种异体重建的T和B淋巴细胞混合物培养物中添加泼尼松龙导致PWM刺激的IgG合成增强。在培养中淋巴细胞数量恒定和增加的情况下均观察到这种效应，与T:B比例无关，并且在含有单核细胞的纯化B淋巴细胞中也会出现。用泼尼松龙预处理含有单核细胞的纯化B淋巴细胞而非纯化T淋巴细胞，可增强T和B淋巴细胞重建混合物中PWM刺激的IgG合成。我们提出，GCS增强人单核细胞经PWM刺激的IgG合成与T淋巴细胞调节无关。
