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欧洲海鲈（Dicentrarchus labrax L.）的微卫星连锁图谱

A microsatellite linkage map of the European sea bass Dicentrarchus labrax L.

作者信息

Chistiakov Dimitry A, Hellemans Bart, Haley Chris S, Law Andy S, Tsigenopoulos Costas S, Kotoulas Georgios, Bertotto Daniela, Libertini Angelo, Volckaert Filip A M

机构信息

Laboratory of Aquatic Ecology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium.

出版信息

Genetics. 2005 Aug;170(4):1821-6. doi: 10.1534/genetics.104.039719. Epub 2005 Jun 3.

DOI:10.1534/genetics.104.039719
PMID:15937133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1449790/
Abstract

A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). A total of 162 microsatellites were mapped in 25 linkage groups. Eleven loci represent type I (coding) markers; 2 loci are located within the peptide Y (linkage group 1) and cytochrome P450 aromatase (linkage group 6) genes. The sex-averaged map spans 814.5 cM of the sea bass genome. The female map covers 905.9 cM, whereas the male map covers only 567.4 cM. The constructed map represents the first linkage map of European sea bass, one of the most important aquaculture species in Europe.

摘要

利用174个微卫星标记构建了欧洲海鲈（Dicentrarchus labrax）的遗传连锁图谱，其中包括本研究报道的145个新标记。作图群体来自亚得里亚海北部养殖的海鲈，由一个包含双亲及50个全同胞后代（双亲二倍体）的家系组成。总共162个微卫星标记被定位到25个连锁群中。11个位点代表I型（编码）标记；2个位点位于肽Y（连锁群1）和细胞色素P450芳香化酶（连锁群6）基因内。性别平均图谱覆盖了海鲈基因组的814.5厘摩。雌性图谱覆盖905.9厘摩，而雄性图谱仅覆盖567.4厘摩。构建的图谱是欧洲海鲈的首张连锁图谱，欧洲海鲈是欧洲最重要的水产养殖物种之一。