Demchenko Alexander P, Duportail Guy, Oncul Sule, Klymchenko Andrey S, Mély Yves
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha Street, Kiev, 01030, Ukraine,
Methods Mol Biol. 2015;1232:19-43. doi: 10.1007/978-1-4939-1752-5_3.
Fluorescence is one of the most powerful and commonly used tools in biophysical studies of biomembrane structure and dynamics that can be applied on different levels, from lipid monolayers and bilayers to living cells, tissues, and whole animals. Successful application of this method relies on proper design of fluorescence probes with optimized photophysical properties. These probes are efficient for studying the microscopic analogs of viscosity, polarity, and hydration, as well as the molecular order, environment relaxation, and electrostatic potentials at the sites of their location. Being smaller than the membrane width they can sense the gradients of these parameters across the membrane. We present examples of novel dyes that achieve increased spatial resolution and information content of the probe responses. In this respect, multiparametric environment-sensitive probes feature considerable promise.
荧光是生物膜结构与动力学的生物物理研究中最强大且常用的工具之一，可应用于从脂质单分子层和双分子层到活细胞、组织及整个动物体等不同层面。该方法的成功应用依赖于具有优化光物理性质的荧光探针的合理设计。这些探针对于研究微观层面的粘度、极性、水合作用以及分子有序性、环境弛豫和其所在位点的静电势非常有效。由于其尺寸小于膜的宽度，它们能够感知这些参数在膜上的梯度变化。我们展示了一些新型染料的实例，这些染料提高了探针响应的空间分辨率和信息含量。在这方面，多参数环境敏感探针具有相当大的前景。
