Adair B M, McNeilly F, McConnell C D, Todd D, Nelson R T, McNulty M S
Department of Agriculture, Veterinary Sciences Division, Stormont, Belfast, Northern Ireland.
Avian Dis. 1991 Oct-Dec;35(4):783-92.
One-day-old chicks with no maternal antibodies to chicken anemia agent (CAA) were inoculated intramuscularly with CAA grown in MDCC-MSB1 cells. A control group of birds from the same source was inoculated intramuscularly with a lysate from uninfected MSB1 cells. Birds were killed at 8, 15, 22, 29, and 43 days postinoculation (PI), and the spleens were removed. Spleen cells were dispersed and stimulated with various concentrations of Concanavalin A (Con A), and lymphocyte transformation responses were determined. Supernatants from Con A-stimulated cultures were assayed for T-cell growth factor (TCGF) and interferon. Decreased lymphocyte transformation and TCGF production were demonstrated at 8 and 15 days PI. This was followed by a stimulation in activities before a return to control levels at 43 days PI. Interferon levels were elevated 8 days after infection. This was followed by a significant decrease in activity compared with controls at 15, 22, and 29 days PI, and a return to control levels by 43 days PI. The results suggest that CAA infection in young chickens can produce a dramatic decrease in immune competence, which, although transitory, is likely to seriously compromise the ability of birds to mount a successful immune response to invading pathogens.
将无鸡贫血因子（CAA）母源抗体的1日龄雏鸡肌肉注射接种在MDCC-MSB1细胞中培养的CAA。来自同一来源的一组对照鸡肌肉注射未感染的MSB1细胞的裂解物。在接种后（PI）8、15、22、29和43天处死鸡，并取出脾脏。将脾细胞分散并用不同浓度的刀豆球蛋白A（Con A）刺激，测定淋巴细胞转化反应。检测Con A刺激培养物的上清液中的T细胞生长因子（TCGF）和干扰素。在接种后8天和15天，淋巴细胞转化和TCGF产生减少。随后在43天接种后恢复到对照水平之前，活性出现刺激。感染后8天干扰素水平升高。随后在接种后15、22和29天，与对照相比活性显著降低，到接种后43天恢复到对照水平。结果表明，幼鸡感染CAA可导致免疫能力急剧下降，尽管这种下降是暂时的，但可能会严重损害鸡对入侵病原体产生成功免疫反应的能力。
