Demeester J, Bracke M, Vochten R, Lauwers A
J Pharm Sci. 1978 May;67(5):729-30. doi: 10.1002/jps.2600670550.
A method is proposed for the simultaneous determination of tyrosine and tryptophan in solutions by differential spectrophotometry. The concentrations are calculated from the measurement of the absorbance of the amino acid mixture in an alkaline medium and from the differential absorbance of the alkaline solution against the acid solution at 294.4 nm. A comparison with three other well-known methods is discussed.
提出了一种通过差示分光光度法同时测定溶液中酪氨酸和色氨酸的方法。根据氨基酸混合物在碱性介质中的吸光度测量值以及碱性溶液相对于酸性溶液在294.4nm处的差示吸光度来计算浓度。讨论了与其他三种知名方法的比较。
