Neri-Cruz Nadia, Gómez-Oliván Leobardo Manuel, Galar-Martínez Marcela, Del Socorro Romero-Figueroa María, Islas-Flores Hariz, García-Medina Sandra, Jiménez-Vargas Juan Manuel, SanJuan-Reyes Nely
Laboratorio de Toxicología Ambiental, Departamento de Farmacia, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120, Toluca, Estado de México, Mexico.
Ecotoxicology. 2015 Jan;24(1):181-93. doi: 10.1007/s10646-014-1371-y. Epub 2014 Oct 22.
The very wide range of activities performed in hospitals (care, diagnosis, hygiene, maintenance, research) require the use of a large variety of potentially ecotoxic substances such as surfactants, metals, disinfectants and pharmaceuticals. This study aimed to determine oxidative stress in the common carp Cyprinus carpio induced by hospital wastewater (HWW) in Mexico. The median lethal concentration (LC50) and subsequently the lowest observed adverse effect level were determined. Carp were exposed to the latter value (0.5 %) for 24, 48, 72 and 96 h, and the following biomarkers were evaluated in gill, brain, liver and blood: hydroperoxide content (HPC), malondialdehyde (MDA) content, protein carbonyl content (PCC) and activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Significant increases in HPC, MDA content and PCC were observed in exposed specimens, particularly in gill, liver and brain. SOD and CAT activity also increased in liver and brain. In conclusion, this particular HWW induces oxidative stress on C. carpio, this damage being most evident in gill, liver and brain.
医院开展的活动范围非常广泛（护理、诊断、卫生、维护、研究），这需要使用种类繁多的潜在生态毒性物质，如表面活性剂、金属、消毒剂和药品。本研究旨在确定墨西哥医院废水（HWW）对鲤鱼（Cyprinus carpio）造成的氧化应激。测定了半数致死浓度（LC50），随后确定了最低观察到的有害作用水平。将鲤鱼暴露于该值（0.5%）24、48、72和96小时，并在鳃、脑、肝脏和血液中评估以下生物标志物：过氧化氢含量（HPC）、丙二醛（MDA）含量、蛋白质羰基含量（PCC）以及抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。在暴露的样本中，尤其是在鳃、肝脏和脑中，观察到HPC、MDA含量和PCC显著增加。肝脏和脑中的SOD和CAT活性也有所增加。总之，这种特定的医院废水会对鲤鱼造成氧化应激，这种损害在鳃、肝脏和脑中最为明显。
