China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):260-266. doi: 10.1099/ijs.0.067231-0. Epub 2014 Oct 21.
A pale yellow, aerobic bacterium, strain M0-2(T), was isolated from a till sample. Its taxonomic position was investigated by using a polyphasic approach. Cells were Gram-stain-negative, rod-shaped and motile. Cells reproduced by budding or asymmetrical cell division. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M0-2(T) belonged to the family Sphingomonadaceae and was closely related to species of the genera Novosphingobium (96.4-92.0%) and Blastomonas (94.6%), Sphingopyxis witflariensis W-50(T) (94.0%), Sphingosinicella soli KSL-125(T) (93.6%) and Sphingomonas astaxanthinifaciens TDMA-17(T) (93.5%). Ubiquinone-10 (Q-10) was the predominant respiratory quinone. The major fatty acids were summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c, 31.9%), summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c, 19.8%) and C14:0 2-OH (8.9%). Sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. Spermidine was the major polyamine observed in the cell. The genomic DNA G+C content was 47.5 mol%. On the basis of phylogenetic relationships and the low DNA G+C content compared with most other genera of the family Sphingomonadaceae, combined with phenotypic and chemotaxonomic data, strain M0-2(T) is considered to represent a novel species in a new genus in the family Sphingomonadaceae for which the name Parablastomonas arctica gen. nov., sp. nov. is proposed. The type strain of Parablastomonas arctica gen. nov., sp. nov. is M0-2(T) ( =CCTCC AB 2012968(T) = NRRL B-59110(T)).
一株浅黄色需氧细菌，M0-2(T) 株，从一块耕土样本中分离得到。通过多相分类法研究了其分类地位。细胞革兰氏染色阴性，杆状，运动。细胞通过出芽或不对称细胞分裂繁殖。基于 16S rRNA 基因序列的系统发育分析表明，菌株 M0-2(T) 属于鞘氨醇单胞菌科，与新鞘氨醇单胞菌属（96.4-92.0%）和 Blastomonas 属（94.6%）、 Sphingopyxis witflariensis W-50(T)（94.0%）、 Sphingosinicella soli KSL-125(T)（93.6%）和 Sphingomonas astaxanthinifaciens TDMA-17(T)（93.5%）的种最为接近。泛醌-10（Q-10）为主要呼吸醌。主要脂肪酸为 sum feature 8（包括 C18:1ω7c 和/或 C18:1ω6c，31.9%）、sum feature 3（包括 C16:1ω7c 和/或 C16:1ω6c，19.8%）和 C14:0 2-OH（8.9%）。鞘糖脂、磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱为主要极性脂。在细胞中观察到的主要多胺是亚精胺。基因组 DNA G+C 含量为 47.5 mol%。基于系统发育关系和与鞘氨醇单胞菌科大多数其他属相比较低的 DNA G+C 含量，结合表型和化学分类学数据，菌株 M0-2(T) 被认为代表了鞘氨醇单胞菌科中的一个新属的新种，命名为北极 Parablastomonas arctica gen. nov.，sp. nov.。北极 Parablastomonas arctica gen. nov.，sp. nov. 的模式菌株为 M0-2(T)（ =CCTCC AB 2012968(T) = NRRL B-59110(T)）。
