Kim MyongChol, Kang OkChol, Zhang Yumin, Ren Lvzhi, Chang Xulu, Jiang Fan, Fang Chengxiang, Zheng Congyi, Peng Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
College of Life Sciences, Kim Il Sung University, Pyongyang, DPR Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):91-100. doi: 10.1099/ijsem.0.000677. Epub 2015 Oct 14.
An orange, Gram-reaction-negative and aerobic bacterium, designated MC 3718T, was isolated from a tundra soil near Ny-Ålesund, Svalbard archipelago, Norway (78° N). The cells were motile with either a polar or a subpolar flagellum and reproduced by budding or asymmetrical cell division. Growth occurred at 4-37 °C (optimum 28-30 °C) and at pH 6.0-10.0 (optimum pH 9.0). Many cells accumulated poly-β-hydroxybutyrate granules and contained a single large polyphosphate granule at a pole or in the middle of the cell. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid, and ubiquinone 10 was the main respiratory quinone. Strain MC 3718T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c; 29.49 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 29.38 %), C17 : 1ω6c (10.15 %), C14 : 0 2-OH (9.05 %) and C16 : 0 (6.84 %) as the major cellular fatty acids. The main polar lipids were two sphingoglycolipids, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unknown phospholipids and two unknown polar lipids. Carotenoids were detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MC 3718T belonged to the family Sphingomonadaceae. The DNA G+C content was 67.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain MC 3718T is considered to represent a novel genus and species in the family Sphingomonadaceae, for which the name Sphingoaurantiacus polygranulatus gen. nov., sp. nov. is proposed. The type strain of Sphingoaurantiacus polygranulatus is MC 3718T ( = CCTCC AB 2014274T = LMG 28636T). Emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii are also provided.
从挪威斯瓦尔巴群岛新奥尔松（北纬78°）附近的苔原土壤中分离出一株橙色、革兰氏阴性需氧细菌，命名为MC 3718T。细胞通过极生或亚极生鞭毛运动，通过出芽或不对称细胞分裂进行繁殖。在4-37 °C（最适温度28-30 °C）和pH 6.0-10.0（最适pH 9.0）条件下生长。许多细胞积累了聚-β-羟基丁酸酯颗粒，并且在细胞的一极或中部含有单个大的多聚磷酸盐颗粒。细胞壁含有内消旋二氨基庚二酸作为诊断性二氨基酸，泛醌10是主要的呼吸醌。菌株MC 3718T的主要细胞脂肪酸包括总和特征3（包含C16 : 1ω7c和/或C16 : 1ω6c；29.49 %）、总和特征8（C18 : 1ω7c和/或C18 : 1ω6c；29.38 %）、C17 : 1ω6c（10.15 %）、C14 : 0 2-OH（9.05 %）和C16 : 0（6.84 %）。主要的极性脂质是两种鞘糖脂、磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油、三种未知的磷脂和两种未知的极性脂质。检测到类胡萝卜素。基于16S rRNA基因序列的系统发育分析表明，菌株MC 3718T属于鞘脂单胞菌科。DNA G+C含量为67.2mol%。基于表型、化学分类和系统发育数据，菌株MC 3718T被认为代表鞘脂单胞菌科中的一个新属和新种，为此提出新属名Sphingoaurantiacus和新种名polygranulatus。多聚颗粒鞘脂橙菌的模式菌株为MC 3718T（=CCTCC AB 2014274T=LMG 28636T）。还提供了对桑德拉金诺拉布菌属、多形杆菌属和根瘤拉布菌属以及嗜湖桑德拉金诺拉布菌、银色根瘤拉布菌和维氏鞘氨醇单胞菌的修订描述。
