Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
J Microbiol. 2018 May;56(5):324-330. doi: 10.1007/s12275-018-7455-2. Epub 2018 May 2.
A pale yellow bacterial strain, designated JJ-A5, was isolated form an agricultural soil from Jeju Island in Republic of Korea. Cells of the strain were Gram-stain-negative, motile, flagellated and rod-shaped. The strain grew at 15-30°C, pH 6.0-9.0, and in the presence of 0-1.5% (w/v) NaCl. Growth occurred on R2A, but not on Luria-Bertani agar, nutrient agar, trypticase soy agar and MacConkey agar. The strain utilized alachlor as a sole carbon source for growth. The strain JJ-A5 showed 16S rRNA gene sequence similarities lower than 95.4% with members of the family Sphingomonadaceae. Phylogenetic analysis showed that the strain belongs to the family Sphingomonadaceae and strain JJ-A5 was distinctly separated from established genera of this family. The strain contained Q-10 as dominant ubiquinone and spermidine as major polyamine. The predominant cellular fatty acids were summed feature 8 (Cω7c and/or Cω6c), summed feature 3 (Cω7c and/or Cω6c), 11-methyl Cω7c, C and C 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, and phosphatidylcholine. The DNA G + C content of the strain was 62.7 mol%. On the basis of the phenotypic, genomic and chemotaxonomic characteristics, strain JJ-A5 is considered to represent a novel genus and species within the family Sphingomonadaceae, for which the name Tardibacter chloracetimidivorans gen. nov., sp. nov. is proposed. The type strain of Tardibacter chloracetimidivorans is JJ-A5 (= KACC 19450 = NBRC 113160).
一株浅黄色的细菌菌株，命名为 JJ-A5，从韩国济州岛的农业土壤中分离得到。该菌株的细胞革兰氏染色阴性，运动，鞭毛和杆状。该菌株在 15-30°C、pH6.0-9.0 和 0-1.5%（w/v）NaCl 存在的条件下生长。该菌株在 R2A 上生长，但不在 Luria-Bertani 琼脂、营养琼脂、胰蛋白酶大豆琼脂和 MacConkey 琼脂上生长。该菌株利用阿特拉津作为生长的唯一碳源。菌株 JJ-A5 的 16S rRNA 基因序列相似性低于 95.4%，与鞘脂单胞菌科的成员相似。系统发育分析表明，该菌株属于鞘脂单胞菌科，菌株 JJ-A5 与该科已建立的属明显分离。该菌株含有 Q-10 作为主要的泛醌和亚精胺作为主要的多胺。主要的细胞脂肪酸为总和特征 8（Cω7c 和/或 Cω6c）、总和特征 3（Cω7c 和/或 Cω6c）、11-甲基 Cω7c、C 和 C2-OH。主要的极性脂为磷脂酰乙醇胺、磷脂酰甘油、神经酰胺糖脂和磷脂酰胆碱。该菌株的 DNA G+C 含量为 62.7 mol%。基于表型、基因组和化学分类学特征，菌株 JJ-A5 被认为代表鞘脂单胞菌科中的一个新属和新种，因此建议使用 Tardibacter chloracetimidivorans gen. nov.，sp. nov. 命名该新属和新种。Tardibacter chloracetimidivorans 的模式菌株为 JJ-A5（=KACC 19450=NBRC 113160）。
