Intensive Care Unit, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Neural Regen Res. 2012 Nov 15;7(32):2500-6. doi: 10.3969/j.issn.1673-5374.2012.32.003.
A sciatic nerve transection and repair model was established in Sprague-Dawley rats by transecting the tendon of obturator internus muscle in the greater sciatic foramen and suturing with nylon sutures. The models were treated with tacrolimus gavage (4 mg/kg per day) for 0, 2, 4 and 6 weeks. Specimens were harvested at 6 weeks of intragastric administration. Masson staining revealed that the collagen fiber content and scar area in the nerve anastomosis of the sciatic nerve injury rats were significantly reduced after tacrolimus administration. Hematoxylin-eosin staining showed that tacrolimus significantly increased myelinated nerve fiber density, average axon diameter and myelin sheath thickness. Intragastric administration of tacrolimus also led to a significant increase in the recovery rate of gastrocnemius muscle wet weight and the sciatic functional index after sciatic nerve injury. The above indices were most significantly improved at 6 weeks after of tacrolimus gavage. The myelinated nerve fiber density in the nerve anastomosis and the sciatic nerve functions had a significant negative correlation with the scar area, as detected by Spearman's rank correlation analysis. These findings indicate that tacrolimus can promote peripheral nerve regeneration and accelerate the recovery of neurological function through the reduction of scar formation.
建立了一种在 SD 大鼠中通过切断大坐骨神经孔中的闭孔内肌肌腱并用尼龙缝线缝合来横断坐骨神经的模型。这些模型用他克莫司灌胃（4mg/kg/天）处理 0、2、4 和 6 周。在灌胃 6 周后采集标本。Masson 染色显示，在坐骨神经损伤大鼠的神经吻合处，他克莫司给药后胶原纤维含量和疤痕面积明显减少。苏木精-伊红染色显示，他克莫司显著增加有髓神经纤维密度、平均轴突直径和髓鞘厚度。他克莫司灌胃还导致坐骨神经损伤后腓肠肌湿重和坐骨神经功能指数的恢复率显著增加。在他克莫司灌胃 6 周后，上述指标的改善最为显著。通过 Spearman 秩相关分析发现，神经吻合处有髓神经纤维密度和坐骨神经功能与疤痕面积呈显著负相关。这些发现表明，他克莫司通过减少疤痕形成可以促进周围神经再生和加速神经功能的恢复。
