Zamani Mohmmad, Katebi M, Mehdizadeh Mehdi, Kafami Laya, Soleimani Mansooreh
Cellular and Molecular Research Center, Tehran University of Medical Sciences.
Persian Gulf Research Center for Stem Cell Therapy of Hormozgan University of Medical Sciences, Bandar Abbas, Iran ; Department of Anatomy, School of Medicine, HormozganUniversity of Medical Sciences.
Basic Clin Neurosci. 2013 Spring;4(2):111-6.
Stroke is one of the most important reasons of death. Hence, trials to prevent or lessen the complications originated by stroke are a goal of public health worldwide. The ischemia-reperfusion causes hypoxia, hypoglycemia and incomplete repel of metabolic waste products and leads to accumulation of free radicals triggering neuronal death. The A1 adenosine receptoras an endogenous ligand of adenosine is known to improve cell resistance to destructive agentsby preventing apoptosis. Vitamin C as a cellular antioxidant is also known as an effective factor to reduce damages initiated by free radicals. We studied the protective effects of A1 receptor agonist in combination with vitamin C against ischemia-reperfusion.
Ischemia was induced by common carotid artery occlusion in bulb-c mice (20-30 gr). Y-Maze was employed to scale the short-term memory and Nissl staining was used to count the cells in hippocampus.
We found that concurrent treatment of A1 receptor agonist and vitamin C significantly reduced neuronal death in CA1. The Memory scores were also significantly improved (P < 0.05).
Our data point to the therapeutic effects of CPA/vitamin C co-administration and highlight the beneficial role of A1 adenosine receptor signaling in the context of stroke.
中风是最重要的死亡原因之一。因此，预防或减轻中风引发的并发症的试验是全球公共卫生的目标。缺血再灌注会导致缺氧、低血糖以及代谢废物排出不完全，从而导致自由基积累，引发神经元死亡。A1腺苷受体作为腺苷的内源性配体，已知可通过防止细胞凋亡来提高细胞对破坏因子的抵抗力。维生素C作为一种细胞抗氧化剂，也是减少自由基引发损伤的有效因素。我们研究了A1受体激动剂与维生素C联合使用对缺血再灌注的保护作用。
通过结扎球囊小鼠（20 - 30克）的颈总动脉诱导缺血。采用Y迷宫评估短期记忆，并用尼氏染色法对海马体中的细胞进行计数。
我们发现，A1受体激动剂与维生素C联合治疗可显著减少CA1区的神经元死亡。记忆分数也显著提高（P < 0.05）。
我们的数据表明CPA/维生素C联合给药具有治疗效果，并突出了A1腺苷受体信号在中风背景下的有益作用。
