Ramos-Oliveira Thayanne Monteiro, Ramos Thaysa Monteiro, Esteves-Oliveira Marcela, Apel Christian, Fischer Horst, Eduardo Carlos de Paula, Steagall Washington, Freitas Patricia Moreira de
Department of Restorative Dentistry, School of Dentistry, Universidade de São Paulo - USP, São Paulo, SP, Brazil.
Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Aachen, Germany.
Braz Oral Res. 2014;28:1-6. doi: 10.1590/1807-3107bor-2014.vol28.0057. Epub 2014 Oct 17.
This in vitro study aimed to investigate the potential of CO2 lasers associated with different fluoride agents in inhibiting enamel erosion. Human enamel samples were randomly divided into 9 groups (n = 12): G1-eroded enamel; G2-APF gel; G3-AmF/NaF gel; G4-AmF/SnF2 solution; G5-CO2 laser (λ = 10.6 µm)+APF gel; G6-CO2 laser+AmF/NaF gel; G7-CO2laser+AmF/SnF2solution; G8-CO2 laser; and G9-sound enamel. The CO2 laser parameters were: 0.45 J/cm2; 6 μs; and 128 Hz. After surface treatment, the samples (except from G9) were immersed in 1% citric acid (pH 4.0, 3 min). Surface microhardness was measured at baseline and after surface softening. The data were statistically analyzed by one-way ANOVA and Tukey's tests (p < 0.05). G2 (407.6 ± 37.3) presented the highest mean SMH after softening, followed by G3 (407.5 ± 29.8) and G5 (399.7 ± 32.9). Within the fluoride-treated groups, G4 (309.0 ± 24.4) had a significantly lower mean SMH than G3 and G2, which were statistically similar to each other. AmF/NaF and APF application showed potential to protect and control erosion progression in dental enamel, and CO2 laser irradiation at 0.45J/cm2 did not influence its efficacy. CO2 laser irradiation alone under the same conditions could also significantly decrease enamel erosive mineral loss, although at lower levels.
这项体外研究旨在探究二氧化碳激光与不同氟化物制剂联合使用时抑制牙釉质侵蚀的潜力。将人类牙釉质样本随机分为9组（n = 12）：G1 - 侵蚀牙釉质组；G2 - APF凝胶组；G3 - AmF/NaF凝胶组；G4 - AmF/SnF₂溶液组；G5 - 二氧化碳激光（λ = 10.6 µm）+APF凝胶组；G6 - 二氧化碳激光+AmF/NaF凝胶组；G7 - 二氧化碳激光+AmF/SnF₂溶液组；G8 - 二氧化碳激光组；以及G9 - 正常牙釉质组。二氧化碳激光参数为：0.45 J/cm²；6 μs；以及128 Hz。表面处理后，样本（G9除外）浸入1%柠檬酸（pH 4.0，3分钟）中。在基线和表面软化后测量表面显微硬度。数据采用单因素方差分析和Tukey检验进行统计学分析（p < 0.05）。软化后，G2组（407.6 ± 37.3）的平均表面显微硬度最高，其次是G3组（407.5 ± 29.8）和G5组（399.7 ± 32.9）。在氟化物处理组中，G4组（309.0 ± 24.4）的平均表面显微硬度显著低于G3组和G2组，而后两组在统计学上彼此相似。应用AmF/NaF和APF显示出保护和控制牙釉质侵蚀进展的潜力，并且0.45J/cm²的二氧化碳激光照射不影响其效果。在相同条件下单独进行二氧化碳激光照射也可显著减少牙釉质侵蚀性矿物质流失，尽管程度较低。
