Cui Shiwei, Li Haibin, Wang Shaojia, Jiang Xiao, Zhang Shusheng, Zhang Rongjie, Sun Xin
Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Department of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan province, China.
Int J Environ Res Public Health. 2014 Oct 21;11(10):10902-14. doi: 10.3390/ijerph111010902.
Etheno-DNA adducts are generated from the metabolism of exogenous carcinogens and endogenous lipid peroxidation. We and others have previously reported that 1,N6-ethenodeoxyadenosine (εdA) and 3,N4-ethenodeoxycytidine (εdC) are present in human urine and can be utilized as biomarkers of oxidative stress. In this study, we report a new ultrasensitive UPLC-ESI-MS/MS method for the analysis of εdA and edC in human urine, capable of detecting 0.5 fmol εdA and 0.3 fmol εdC in 1.0 mL of human urine, respectively. For validation of the method, 20 human urine samples were analyzed, and the results revealed that the mean levels of εdA and εdC (SD) fmol/µmol creatinine are 5.82 ± 2.11 (range 3.0-9.5) for εdA and 791.4 ± 328.8 (range 116.7-1264.9) for εdC in occupational benzene-exposed workers and 2.10 ± 1.32 (range 0.6-4.7) for εdA and 161.8 ± 200.9 (range 1.8-557.5) for εdC in non-benzene-exposed workers, respectively. The ultrasensitive detection method is thus suitable for applications in human biomonitoring and molecular epidemiology studies.
乙烯基-DNA加合物由外源性致癌物的代谢和内源性脂质过氧化作用产生。我们和其他研究人员之前曾报道，1,N6-乙烯基脱氧腺苷（εdA）和3,N4-乙烯基脱氧胞苷（εdC）存在于人类尿液中，可作为氧化应激的生物标志物。在本研究中，我们报告了一种用于分析人类尿液中εdA和εdC的新型超灵敏超高效液相色谱-电喷雾串联质谱法，该方法能够分别检测1.0 mL人类尿液中的0.5 fmol εdA和0.3 fmol εdC。为验证该方法，分析了20份人类尿液样本，结果显示，职业性接触苯的工人尿液中εdA和εdC的平均水平（标准差）分别为5.82±2.11（范围3.0 - 9.5）fmol/µmol肌酐和791.4±328.8（范围116.7 - 1264.9）fmol/µmol肌酐，而未接触苯的工人尿液中εdA和εdC的平均水平分别为2.10±1.32（范围0.6 - 4.7）fmol/µmol肌酐和161.8±200.9（范围1.8 - 557.5）fmol/µmol肌酐。因此，这种超灵敏检测方法适用于人类生物监测和分子流行病学研究。
