Xiao Tongqian, Lu Cuiyun, Xu Yulan, Li Chao, Zheng Xianhu, Cao Dingchen, Cheng Lei, Mahboob Shahid, Sun Xiaowen
Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, No. 232 Hesong Road, Daoli District, Harbin, 150070, Heilongjiang, China.
J Appl Genet. 2015 May;56(2):261-9. doi: 10.1007/s13353-014-0250-9. Epub 2014 Oct 23.
Fish scale is an attractive model in bone physiology research and is also a crucial character for breeding new varieties. Thus, it is important to identify loci in the genome associated with scale formation. In this study, 290 microsatellite markers in common carp (Cyprinus carpio L.) were selected and tested for their segregation in a full-sib mapping panel containing 96 individuals (population 1). Association analysis identified seven simple sequence repeats (SSRs) (HLJ2509, HLJ3227, HLJ3675, HLJ3766, HLJ3863, FGFR1, FGFR7) that showed significant correlation with scale cover pattern in population 1. When the seven SSRs were investigated in two other populations, seven and five SSRs were significantly correlated with scale cover pattern in population 2 (116 individuals) and population 3 (57 individuals), respectively. The exceptions were FGFR1 and HLJ3227. A genetic linkage map was constructed using the 290 SSRs and 241 SSRs were mapped into 47 linkage groups (LGs), with 2-15 markers per LG. The map spanned 2,241.7 cM, with LG sizes that varied from 1.1 to 124.9 cM. All seven markers associated with scale cover mapped into LG3. We considered that a gene cluster that affected the scale cover pattern possibly existed in LG3. By aligning the seven markers with the zebrafish (Danio rerio) genome, we identified six candidate genes (atoh1a, ptch1, bmp1a, fgfr1a, fgf17, wnt5a) that may be associated with scale formation. We propose that the seven markers could be used with marker-assisted selection to breed a new variety of common carp, and the six candidate genes could help in understanding the scale cover mechanism.
鱼鳞是骨生理学研究中一个有吸引力的模型，也是培育新品种的关键特征。因此，识别基因组中与鳞片形成相关的位点很重要。在本研究中，选择了290个鲤鱼（Cyprinus carpio L.）微卫星标记，并在一个包含96个个体的全同胞作图群体（群体1）中测试它们的分离情况。关联分析确定了7个简单序列重复（SSR）（HLJ2509、HLJ3227、HLJ3675、HLJ3766、HLJ3863、FGFR1、FGFR7）与群体1中的鳞片覆盖模式显著相关。当在另外两个群体中研究这7个SSR时，分别有7个和5个SSR与群体2（116个个体）和群体3（57个个体）中的鳞片覆盖模式显著相关。例外的是FGFR1和HLJ3227。使用这290个SSR构建了遗传连锁图谱，241个SSR被定位到47个连锁群（LG）中，每个LG有2 - 15个标记。该图谱跨度为2241.7 cM，LG大小从1.1到124.9 cM不等。所有与鳞片覆盖相关的7个标记都定位到了LG3中。我们认为LG3中可能存在一个影响鳞片覆盖模式的基因簇。通过将这7个标记与斑马鱼（Danio rerio）基因组进行比对，我们确定了6个可能与鳞片形成相关的候选基因（atoh1a、ptch1、bmp1a、fgfr1a、fgf17、wnt5a）。我们建议这7个标记可用于标记辅助选择来培育鲤鱼新品种，这6个候选基因有助于理解鳞片覆盖机制。
