Small. 2014 Sep 10;10(17):3579-87. doi: 10.1002/smll.201303218.
A biosensor chip is developed for the detection of a protein biomarker of endocrine disrupting compounds, vitellogenin (Vg) in aquatic environment. The sensor chip is fabricated by immobilizing anti-Vg antibody on 4-Aminothiophenol (4-ATP) coated nanosculptured thin films (nSTFs) of silver on Si substrates. The biosensor is based on the SERS of 4-ATP, enhanced by the Ag nSTFs. Before the fabrication of the sensor, the performance of the enhancement is optimized with respect to the porosity of nSTFs. Further, the biosensor is developed on the nSTF with optimized enhancement. The SERS signals are recorded from the sensor chip for varying concentrations of Vg. A control experiment is performed on another similar protein Fetuin to confirm the specificity of the sensor. The repeatability and reusability of the sensor, along with its shelf life are also checked. The limit of detection of the sensor is found to be 5 pg mL −1 of Vg in PBS within our experimental window. Apart from high sensitivity, specificity and reusability, the present sensor provides additional advantages of miniaturization, requirement of very small volumes of the analyte solution (15 μL) and fast response as compared to conventional techniques e.g., ELISA, as its response time is less than 3 minutes.
一种用于检测水生环境内分泌干扰化合物生物标志物卵黄蛋白原 (Vg) 的生物传感器芯片被开发出来。该传感器芯片通过将抗 Vg 抗体固定在 Si 衬底上的银纳米雕刻薄膜 (nSTFs) 上的 4-氨基噻吩 (4-ATP) 上制造而成。该生物传感器基于 4-ATP 的 SERS，通过 Ag nSTFs 增强。在制造传感器之前，针对 nSTFs 的多孔性对增强性能进行了优化。此外，在优化后的增强效果的 nSTF 上开发了生物传感器。从传感器芯片记录不同浓度 Vg 的 SERS 信号。在另一种类似的蛋白质胎球蛋白上进行对照实验，以确认传感器的特异性。还检查了传感器的可重复性、可重复使用性和保质期。发现传感器的检测限在 PBS 中为 5 pg mL-1 的 Vg，在我们的实验窗口内。与传统技术（例如 ELISA）相比，除了高灵敏度、特异性和可重复使用性之外，本传感器还具有小型化、对分析物溶液体积要求非常小（15 μL）和快速响应的额外优势，其响应时间小于 3 分钟。
