Leite Diego Portes Vieira, Paolillo Fernanda Rossi, Parmesano Thiago Nogueira, Fontana Carla Raquel, Bagnato Vanderlei Salvador
1 Department of Biomedical Engineering from University of Camilo Castelo Branco (UNICASTELO) São José dos Campos , SP, Brazil .
Photomed Laser Surg. 2014 Nov;32(11):627-32. doi: 10.1089/pho.2014.3805. Epub 2014 Oct 24.
The purpose of this study was to evaluate the effects of the antimicrobial photodynamic therapy (a-PDT) with blue light and curcumin on oral disinfection during the 2 h after treatment.
a-PDT is a technique that can potentially affect the viability of bacterial cells, with selective action targeting only areas with photosensitizer accumulation.
A randomized controlled trial was undertaken. Twenty-seven adults were randomly divided into three groups: (1) the PDT group, which was treated with the drug, curcumin, and blue light (n=9); (2) the light group, which was treated only with the blue light, and no drug (n=9) and; (3) the curcumin group, which was treated only with the drug, curcumin, and no light (n=9). The irradiation parameters were: blue light-emitting diode (LED) illumination (455±30 nm), 400 mW of average optical power, 5 min of application, illumination area of 0.6 cm(2), 600 mW/cm(2) of intensity, and 200 J/cm(2) of fluence. A curcumin concentration of 30 mg/L was used. The saliva samples were collected for bacterial counts at baseline and after the experimental phases (immediately after treatment, and 1 and 2 h after treatment). Serial dilutions were performed, and the resulting samples were cultured on blood agar plates in microaerophilic conditions. The number of colony-forming units (CFU) was determined.
The PDT group showed a significant reduction of CFU immediately after treatment (post-treatment) with PDT (5.71±0.48, p=0.001), and 1 h (5.14±0.92, p=0.001) and 2 h (5.35±0.76, p=0.001) after treatment, compared with pretreatment (6.61±0.82). There were no significant changes for the light group. The curcumin group showed a significant increase of CFU 1 h after treatment (6.77±0.40, p=0.02) compared with pretreatment (5.57±0.91) falling to baseline values at 2 h after treatment (5.58±0.70).
The PDT group showed significant difference in microbial reduction (p<0.05) compared with both the light and curcumin groups until 2 h post-treatment. The new blue LED device for PDT using curcumin may be used for reduction of salivary microorganisms, leading to overall disinfection of the mouth (e.g., mucosa, tongue, and saliva), but new protocols should be explored.
本研究旨在评估蓝光联合姜黄素的抗菌光动力疗法（a-PDT）在治疗后2小时内对口腔消毒的效果。
a-PDT是一种可能影响细菌细胞活力的技术，其选择性作用仅针对有光敏剂积聚的区域。
进行了一项随机对照试验。27名成年人被随机分为三组：（1）PDT组，用姜黄素药物和蓝光治疗（n=9）；（2）光组，仅用蓝光治疗，不使用药物（n=9）；（3）姜黄素组，仅用姜黄素药物治疗，不使用光（n=9）。照射参数为：蓝光发光二极管（LED）照明（455±30nm），平均光功率400mW，照射时间5分钟，照射面积0.6cm²，强度600mW/cm²，通量200J/cm²。使用的姜黄素浓度为30mg/L。在基线以及实验阶段后（治疗后立即、治疗后1小时和2小时）收集唾液样本进行细菌计数。进行系列稀释，然后将所得样本在微需氧条件下的血琼脂平板上培养。测定菌落形成单位（CFU）的数量。
与治疗前（6.61±0.82）相比，PDT组在PDT治疗后立即（治疗后）CFU显著降低（5.71±0.48，p=0.001），治疗后1小时（5.14±0.92，p=0.001）和2小时（5.35±0.76，p=0.001）也显著降低。光组无显著变化。与治疗前（5.57±0.91）相比，姜黄素组在治疗后1小时CFU显著增加（6.77±0.40，p=0.02），治疗后2小时降至基线值（5.58±0.70）。
与光组和姜黄素组相比，直到治疗后2小时，PDT组在微生物减少方面有显著差异（p<0.05）。使用姜黄素的新型蓝光LED PDT装置可用于减少唾液中的微生物，从而实现口腔（如黏膜、舌头和唾液）的整体消毒，但应探索新的方案。
