School of Physical Education, State University of Minas Gerais (UEMG), R. Colorado, 700 - Bairro São Francisco, Passos, MG, CEP 37902-092, Brazil.
Motricity Science Institute, Rehabilitation Science Program from Federal University of Alfenas (UNIFAL), Av. Jovino Fernandes Sales, 2600 - Santa Clara, Alfenas, MG, CEP: 37133-840, Brazil.
Lasers Med Sci. 2021 Aug;36(6):1219-1226. doi: 10.1007/s10103-020-03160-6. Epub 2020 Oct 16.
Healing wounds represent a major public health problem, mainly when it is infected. Besides that, the antibiotics misuse and overuse favor the development of bacterial resistance. This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with artificial skin on disinfection of infected skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n = 6): (i) control-untreated; (ii) aPDT-treated with curcumin-mediated aPDT (blue light); (iii) artificial skin-treated with artificial skin alcohol-based; and (iv) aPDT plus artificial skin-treated with aPDT associated with artificial skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0.80 cm was performed in order to inoculate the microorganism Staphylococcus aureus (ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm) at the dose of 60 J/cm and the treatment with alcohol-based artificial skin was done with the topical application of 250 μL. Additional animals were submitted to aPDT combined with the artificial skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability was observed in the PDT plus artificial skin group (4.14 log10), followed by artificial skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for artificial skin, and 35.02% for PDT plus artificial) in comparison with the control group. These findings provide evidence for the positive benefits of aPDT with blue light and curcumin associated with artificial skin to decontaminate and accelerate the wound contraction.
治愈伤口是一个主要的公共卫生问题，特别是当伤口感染时。此外，抗生素的滥用和过度使用有利于细菌耐药性的发展。本研究评估了抗菌光动力疗法（aPDT）联合人工皮肤对大鼠感染皮肤伤口消毒的效果。将 24 只 Wistar 大鼠随机分为 4 组（n = 6）：（i）未治疗对照组；（ii）用姜黄素介导的 aPDT（蓝光）治疗的 aPDT 组；（iii）用含酒精的人工皮肤治疗的人工皮肤组；（iv）用 aPDT 联合含酒精的人工皮肤治疗的 aPDT 加人工皮肤组。对于体内模型，进行了 0.80 cm 的全层活检，以接种金黄色葡萄球菌（ATCC 25923）微生物。aPDT 用姜黄素凝胶和 450nm、80mW/cm 的蓝光 LED 灯进行，剂量为 60 J/cm，用 250μL 的含酒精的人工皮肤进行治疗。对额外的动物进行 aPDT 联合人工皮肤治疗。治疗后，确定菌落形成单位（CFU）数量和损伤面积。采用双向重复测量方差分析和 Tukey 检验对数据进行分析。在 PDT 加人工皮肤组观察到细菌活力的最大降低（4.14 log10），其次是人工皮肤组（2.38 log10）和 PDT 组（2.22 log10）。此外，与对照组相比，所有治疗组的伤口收缩相对面积均较高（PDT 为 36.21%，人工皮肤为 38.41%，PDT 加人工皮肤为 35.02%）。这些发现为蓝光和姜黄素联合人工皮肤的 aPDT 对去污和加速伤口收缩的积极益处提供了证据。
