Roda Aldo, Guardigli Massimo, Calabria Donato, Calabretta Maria Maddalena, Cevenini Luca, Michelini Elisa
Laboratory of Analytical and Bioanalytical Chemistry, Department of Chemistry "G. Ciamician", Alma Mater Studiorum - University of Bologna, Via Selmi 2, Bologna, 40126, Italy.
Analyst. 2014 Dec 21;139(24):6494-501. doi: 10.1039/c4an01612b.
Increasingly, smartphones are used as portable personal computers, revolutionizing communication styles and entire lifestyles. Using 3D-printing technology we have made a disposable minicartridge that can be easily prototyped to turn any kind of smartphone or tablet into a portable luminometer to detect chemiluminescence derived from enzyme-coupled reactions. As proof-of-principle, lactate oxidase was coupled with horseradish peroxidase for lactate determination in oral fluid and sweat. Lactate can be quantified in less than five minutes with detection limits of 0.5 mmol L(-1) (corresponding to 4.5 mg dL(-1)) and 0.1 mmol L(-1) (corresponding to 0.9 mg dL(-1)) in oral fluid and sweat, respectively. A smartphone-based device shows adequate analytical performance to offer a cost-effective alternative for non-invasive lactate measurement. It could be used to evaluate lactate variation in relation to the anaerobic threshold in endurance sport and for monitoring lactic acidosis in critical-care patients.
智能手机越来越多地被用作便携式个人电脑，彻底改变了通信方式和整个生活方式。我们利用3D打印技术制作了一种一次性微型盒，它可以很容易地被制作成原型，将任何类型的智能手机或平板电脑变成便携式发光计，以检测酶联反应产生的化学发光。作为原理验证，乳酸氧化酶与辣根过氧化物酶偶联用于测定口腔液和汗液中的乳酸。在口腔液和汗液中，分别可在不到五分钟的时间内对乳酸进行定量，检测限分别为0.5 mmol L(-1)（相当于4.5 mg dL(-1)）和0.1 mmol L(-1)（相当于0.9 mg dL(-1)）。基于智能手机的设备显示出足够的分析性能，可为非侵入性乳酸测量提供一种经济高效的替代方案。它可用于评估耐力运动中与无氧阈值相关的乳酸变化，以及监测重症患者的乳酸性酸中毒。
