用于荧光引导癌症手术的阿尔忒弥斯相机的特性与评估
Characterization and evaluation of the artemis camera for fluorescence-guided cancer surgery.
作者信息
van Driel P B A A, van de Giessen M, Boonstra M C, Snoeks T J A, Keereweer S, Oliveira S, van de Velde C J H, Lelieveldt B P F, Vahrmeijer A L, Löwik C W G M, Dijkstra J
机构信息
Department of Radiology and Molecular Imaging, Leiden University Medical Center, Leiden, The Netherlands.
出版信息
Mol Imaging Biol. 2015 Jun;17(3):413-23. doi: 10.1007/s11307-014-0799-z.
PURPOSE
Near-infrared (NIR) fluorescence imaging can provide the surgeon with real-time visualization of, e.g., tumor margins and lymph nodes. We describe and evaluate the Artemis, a novel, handheld NIR fluorescence camera.
PROCEDURES
We evaluated minimal detectable cell numbers (FaDu-luc2, 7D12-IRDye 800CW), preclinical intraoperative detection of sentinel lymph nodes (SLN) using indocyanine green (ICG), and of orthotopic tongue tumors using 7D12-800CW. Results were compared with the Pearl imager. Clinically, three patients with liver metastases were imaged using ICG.
RESULTS
Minimum detectable cell counts for Artemis and Pearl were 2 × 10(5) and 4 × 10(4) cells, respectively. In vivo, seven SLNs were detected in four mice with both cameras. Orthotopic OSC-19-luc2-cGFP tongue tumors were clearly identifiable, and a minimum FaDu-luc2 tumor size of 1 mm(3) could be identified. Six human malignant lesions were identified during three liver surgery procedures.
CONCLUSIONS
Based on this study, the Artemis system has demonstrated its utility in fluorescence-guided cancer surgery.
目的
近红外（NIR）荧光成像可为外科医生提供例如肿瘤边缘和淋巴结的实时可视化图像。我们描述并评估了一种新型手持式NIR荧光相机Artemis。
程序
我们评估了最小可检测细胞数（FaDu-luc2，7D12-IRDye 800CW），使用吲哚菁绿（ICG）进行临床前术中前哨淋巴结（SLN）检测，以及使用7D12-800CW对原位舌肿瘤进行检测。将结果与Pearl成像仪进行比较。临床上，对3例肝转移患者使用ICG进行成像。
结果
Artemis和Pearl的最小可检测细胞数分别为2×10⁵和4×10⁴个细胞。在体内，两台相机在4只小鼠中均检测到7个SLN。原位OSC-19-luc2-cGFP舌肿瘤清晰可辨，并且可以识别出最小尺寸为1 mm³的FaDu-luc2肿瘤。在3例肝脏手术过程中识别出6例人类恶性病变。
结论
基于本研究，Artemis系统已证明其在荧光引导癌症手术中的效用。
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