Lovreglio Piero, Maffei Francesca, Carrieri Mariella, D'Errico Maria N, Drago Ignazio, Hrelia Patrizia, Bartolucci Giovanni B, Soleo Leonardo
Interdisciplinary Department of Medicine, Section of Occupational Medicine "E.C. Vigliani", University of Bari, Bari, Italy.
Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, Rimini, Italy.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Aug;770:55-60. doi: 10.1016/j.mrgentox.2014.04.022. Epub 2014 Jun 2.
The frequency of chromosome aberrations (CA) and micronuclei (MN) was investigated in the peripheral lymphocytes of workers occupationally exposed to low or very low concentrations of benzene. The study included 43 exposed workers (all males), namely 19 fuel-tanker drivers and 24 filling-station attendants, and 31 male subjects with no occupational exposure to the toxicant (controls). Benzene exposure was verified by means of environmental monitoring with passive personal samplers (Radiello(®)), and through biological monitoring, i.e. by measurement of urinary trans,trans-muconic acid, S-phenylmercapturic acid and benzene. The frequency of CA and MN in peripheral lymphocytes was determined according to standard procedures. Exposure to benzene was found to be significantly higher for fuel-tanker drivers (median 246.6 μg/m(3)) than for filling-station attendants (median 19.9 μg/m(3)). Both groups had significantly higher exposure than controls (median 4.3 μg/m(3)). No increased frequency of CA and MN was observed in either fuel-tanker drivers or filling-station attendants compared with controls. In all subjects examined as a single group, the frequency of MN was significantly dependent on age. Only in the fuel-tanker drivers was the frequency of MN found to depend not only on age, but also on exposure to benzene. In conclusion, the frequency of MN, but not of CA, could be influenced by exposure to benzene concentrations of up to one order of magnitude lower than the threshold limit value (time-weighted average).
对职业性接触低浓度或极低浓度苯的工人外周血淋巴细胞中的染色体畸变（CA）和微核（MN）频率进行了调查。该研究纳入了43名接触苯的工人（均为男性），即19名油罐车司机和24名加油站工作人员，以及31名未职业接触该毒物的男性受试者（对照组）。通过使用被动式个人采样器（Radiello(®)）进行环境监测以及通过生物监测，即测量尿中反式、反式粘康酸、S - 苯巯基尿酸和苯，来验证苯接触情况。根据标准程序测定外周血淋巴细胞中CA和MN的频率。发现油罐车司机的苯接触水平（中位数为246.6μg/m³）显著高于加油站工作人员（中位数为19.9μg/m³）。两组的接触水平均显著高于对照组（中位数为4.3μg/m³）。与对照组相比，油罐车司机和加油站工作人员的CA和MN频率均未增加。在作为一个单一组检查的所有受试者中，MN频率显著依赖于年龄。仅在油罐车司机中发现MN频率不仅依赖于年龄，还依赖于苯接触。总之，MN频率而非CA频率可能受到接触低于阈限值（时间加权平均值）一个数量级的苯浓度的影响。
