Göethel Gabriela, Brucker Natália, Moro Angela M, Charão Mariele F, Fracasso Rafael, Barth Anelise, Bubols Guilherme, Durgante Juliano, Nascimento Sabrina, Baierle Marília, Saldiva Paulo H, Garcia Solange C
Laboratory of Toxicology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Post-graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Post-graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Aug;770:61-5. doi: 10.1016/j.mrgentox.2014.05.008. Epub 2014 Jun 4.
Gas station attendants and taxi drivers are occupationally exposed to xenobiotics which may be harmful to their health. Atmospheric pollutants and benzene can lead to DNA damage. Genotoxicity and mutagenicity assays can be used to evaluate the effects of these pollutants. We have evaluated genotoxicity and mutagenicity in workers occupationally exposed to xenobiotics, by application of the 8-hydroxy-2-deoxyguanosine (8-OHdG), comet, and micronucleus (MN) assays. Biomarkers of benzene and carbon monoxyde exposure were also measured: urinary t,t-muconic acid (t,t-MA) and carboxyhaemoglobin (COHb) in whole blood, respectively. The study groups comprised 43 gas station attendants (GSA), 34 taxi drivers (TD), and 22 persons without known occupational exposures (NE). Levels of t,t-MA in the GSA group were significantly elevated compared to the NE group (p<0.001), however these levels were below of levels established by ACGIH (American Conference of Governmental Industrial Hygienists). COHb levels were not significantly different between the TD and NE groups (p>0.05). DNA damage index (DI) and 8-OHdG levels were significantly higher for both the GSA and TD groups, compared to the NE group (p<0.001), but MN frequencies were not elevated. Spearman correlation analysis showed that the frequency of MN was positively correlated with 8-OHdG. A positive correlation between DNA DI levels and 8-OHdG was also observed. In conclusion, our results indicated that low levels of occupational exposure to benzene and atmospheric pollutants may be linked to genotoxicity and oxidative DNA damage.
加油站工作人员和出租车司机职业性地接触可能对其健康有害的外源性物质。大气污染物和苯会导致DNA损伤。遗传毒性和致突变性检测可用于评估这些污染物的影响。我们通过应用8-羟基-2-脱氧鸟苷（8-OHdG）、彗星试验和微核（MN）试验，评估了职业性接触外源性物质的工人的遗传毒性和致突变性。还测量了苯和一氧化碳暴露的生物标志物：分别为尿中反,反-粘糠酸（t,t-MA）和全血中的碳氧血红蛋白（COHb）。研究组包括43名加油站工作人员（GSA）、34名出租车司机（TD）和22名无已知职业暴露的人员（NE）。与NE组相比，GSA组的t,t-MA水平显著升高（p<0.001），然而这些水平低于美国政府工业卫生学家会议（ACGIH）确定的水平。TD组和NE组之间的COHb水平无显著差异（p>0.05）。与NE组相比，GSA组和TD组的DNA损伤指数（DI）和8-OHdG水平均显著更高（p<0.001），但微核频率未升高。Spearman相关性分析表明，微核频率与8-OHdG呈正相关。还观察到DNA DI水平与8-OHdG之间呈正相关。总之，我们的结果表明，低水平的职业性苯和大气污染物暴露可能与遗传毒性和氧化性DNA损伤有关。
