Iqtedar Mehwish, Nadeem Mohammad, Naeem Hira, Abdullah Roheena, Naz Shagufta, Kaleem Afshan
a Department of Biotechnology , Lahore College for Women University , Lahore , Pakistan.
Nat Prod Res. 2015;29(11):1012-9. doi: 10.1080/14786419.2014.971320. Epub 2014 Oct 27.
The industrialisation of lignocellulose conversion is impeded by expensive cellulase enzymes required for saccharification in bioethanol production. Current research undertakes cellulase production from pretreated Saccharum spontaneum through Trichoderma viride HN1 under submerged fermentation conditions. Pretreatment of substrate with 2% NaOH resulted in 88% delignification. Maximum cellulase production (2603 ± 16.39 U/mL/min carboxymethyl cellulase and 1393 ± 25.55 U/mL/min FPase) was achieved at 6% substrate at pH 5.0, with 5% inoculum, incubated at 35°C for 120 h of fermentation period. Addition of surfactant, Tween 80 and metal ion Mn(+2), significantly enhanced cellulase yield. This study accounts proficient cellulase yield through process optimisation by exploiting cheaper substrate to escalate their commercial endeavour.
木质纤维素转化的工业化进程受到生物乙醇生产糖化所需的昂贵纤维素酶的阻碍。目前的研究是在深层发酵条件下，通过绿色木霉HN1从预处理的甜根子草中生产纤维素酶。用2%的氢氧化钠对底物进行预处理，脱木素率达88%。在底物浓度为6%、pH值为5.0、接种量为5%、35℃下培养120小时的发酵周期时，纤维素酶产量达到最高（羧甲基纤维素酶为2603±16.39 U/mL/分钟，滤纸酶为1393±25.55 U/mL/分钟）。添加表面活性剂吐温80和金属离子锰（+2）可显著提高纤维素酶产量。本研究通过利用更廉价的底物进行工艺优化，以提高纤维素酶产量，从而推动其商业应用。
