Zhu Qijun, Liu Weiwei, Song Liye, Guo Zhenzhen, Bian Zhiyao, Han Yunsheng, Cai Hongying, Yang Peilong, Meng Kun
Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
Front Microbiol. 2025 Apr 23;16:1550495. doi: 10.3389/fmicb.2025.1550495. eCollection 2025.
This study explored 's lignocellulose degradation potential in wheat straw (WS) and NaOH-treated WS via solid-state fermentation (SSF) over 30 days. Compared to the control, WS treated with (TW) and NaOH-treated WS with (TN) showed increased dry matter loss rates of 15.67 and 15.76%, respectively. Cellulose degradation reached 33.51 and 28.00%, while hemicellulose degradation increased to 31.56 and 63.86%. Crude protein (CP) content rose to 10.96 and 7.44%, and reducing sugar content to 10.86 and 12.41 mg/g, respectively. effectively reduced lignocellulose content and enhanced substrate nutrition, supporting subsequent uses of WS as fertilizer, feed, or for bioethanol production. Enzymatic activity and structural analyses were performed to further confirm the lignocellulose-degrading ability of and to analyze the degradation mechanisms. Transcriptomic analysis revealed that, compared with the control group, the TN group had 4,548, 4,399, and 6,051 differentially expressed genes (DEGs) at 5, 10, and 30 days, respectively, mainly involved in cellulose and hemicellulose degradation, carbohydrate metabolism, carbohydrate transport, glycoside hydrolases, and polysaccharide binding. can modify lignin by expressing dye-decolorizing peroxidase genes, and multiple key genes were identified for further research into its genetic regulation in lignocellulose degradation.
本研究通过固态发酵（SSF）在30天内探索了[具体名称未给出]对小麦秸秆（WS）和氢氧化钠处理的WS的木质纤维素降解潜力。与对照组相比，用[具体名称未给出]处理的WS（TW）和用[具体名称未给出]处理的氢氧化钠处理的WS（TN）的干物质损失率分别提高了15.67%和15.76%。纤维素降解率分别达到33.51%和28.00%，而半纤维素降解率分别提高到31.56%和63.86%。粗蛋白（CP）含量分别升至10.96%和7.44%，还原糖含量分别升至10.86和12.41mg/g。[具体名称未给出]有效降低了木质纤维素含量并提高了底物营养，支持了WS后续用作肥料、饲料或用于生物乙醇生产。进行了酶活性和结构分析，以进一步确认[具体名称未给出]的木质纤维素降解能力并分析降解机制。转录组分析表明，与对照组相比，TN组在第5、10和30天分别有4548、4399和6051个差异表达基因（DEG），主要涉及纤维素和半纤维素降解、碳水化合物代谢、碳水化合物转运、糖苷水解酶和多糖结合。[具体名称未给出]可以通过表达染料脱色过氧化物酶基因来修饰木质素，并鉴定了多个关键基因，以便进一步研究其在木质纤维素降解中的遗传调控。
