Kotz Kenneth T, Petrofsky Anne C, Haghgooie Ramin, Granier Robert, Toner Mehmet, Tompkins Ronald G
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children, Boston, MA 02114, USA.
Technology (Singap World Sci). 2013;1(1):27-36. doi: 10.1142/S233954781350009X.
Microfluidic inertial focusing has been shown as a simple and effective method to localize cells and particles within a flow cell for interrogation by an external optical system. To enable portable point of care optical cytometry, however, requires a reduction in the complexity of the large optical systems that are used in standard flow cytometers. Here, we present a new design that incorporates optical waveguides and focusing elements with an inertial focusing flow cell to make a compact robust cytometer capable of enumerating and discriminating beads, cells, and platelets.
微流控惯性聚焦已被证明是一种简单有效的方法，可将细胞和颗粒定位在流动池中，以便由外部光学系统进行检测。然而，要实现便携式即时光学细胞计数，需要降低标准流式细胞仪中使用的大型光学系统的复杂性。在此，我们提出一种新设计，将光波导和聚焦元件与惯性聚焦流动池相结合，制成一种紧凑坚固的细胞计数器，能够对珠子、细胞和血小板进行计数和鉴别。
